Enzyme linked immunosorbent assay in analysis of deoxynivalenol: Investigation of impact of sample matrix on results accuracy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43898065" target="_blank" >RIV/60461373:22330/14:43898065 - isvavai.cz</a>

Výsledek na webu

<a href="http://download.springer.com/static/pdf/636/art%253A10.1007%252Fs00216-013-7463-3.pdf?auth66=1423854967_9b6a3b3cc61e44e01f576199fa388794&ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/636/art%253A10.1007%252Fs00216-013-7463-3.pdf?auth66=1423854967_9b6a3b3cc61e44e01f576199fa388794&ext=.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-013-7463-3" target="_blank" >10.1007/s00216-013-7463-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enzyme linked immunosorbent assay in analysis of deoxynivalenol: Investigation of impact of sample matrix on results accuracy

Popis výsledku v původním jazyce

Enzyme-linked immunosorbent assay (ELISA) represents a bioanalytical strategy frequently used for rapid screening of mycotoxin deoxynivalenol (DON) in cereals and derived products. Due to a considerable affinity of some anti-DON antibodies to structurally similar DON metabolites, such as DON-3-glucoside (DON-3-Glc) and 3-acetyl-DON (3-ADON), a significant overestimation of DON concentrations may occur. A validation study of six commercial DON-dedicated ELISA kits, namely Ridascreen DON, Ridascreen FASTDON, DON EIA, AgraQuant DON Assay, Veratox 5/5 and Veratox HS was carried out on wheat, barley and malt matrices. Performance characteristics of all tested ELISAs were determined using aqueous solutions of DON, DON-3-Glc and 3-ADON analytical standards,further with extracts of artificially spiked blank cereals and finally with matrix-matched standards of all three compounds. In the final phase, the accuracy of data was assessed through a comparison of DON concentrations determined by pa

Název v anglickém jazyce

Enzyme linked immunosorbent assay in analysis of deoxynivalenol: Investigation of impact of sample matrix on results accuracy

Popis výsledku anglicky

Enzyme-linked immunosorbent assay (ELISA) represents a bioanalytical strategy frequently used for rapid screening of mycotoxin deoxynivalenol (DON) in cereals and derived products. Due to a considerable affinity of some anti-DON antibodies to structurally similar DON metabolites, such as DON-3-glucoside (DON-3-Glc) and 3-acetyl-DON (3-ADON), a significant overestimation of DON concentrations may occur. A validation study of six commercial DON-dedicated ELISA kits, namely Ridascreen DON, Ridascreen FASTDON, DON EIA, AgraQuant DON Assay, Veratox 5/5 and Veratox HS was carried out on wheat, barley and malt matrices. Performance characteristics of all tested ELISAs were determined using aqueous solutions of DON, DON-3-Glc and 3-ADON analytical standards,further with extracts of artificially spiked blank cereals and finally with matrix-matched standards of all three compounds. In the final phase, the accuracy of data was assessed through a comparison of DON concentrations determined by pa

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Svazek periodika

406

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

505-514

Kód UT WoS článku

000329626600014

EID výsledku v databázi Scopus

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