MALDI-TOF MS identification of spoilage microorganisms in dairy industry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43899338" target="_blank" >RIV/60461373:22330/14:43899338 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

MALDI-TOF MS identification of spoilage microorganisms in dairy industry

Popis výsledku v původním jazyce

The fast and reliable identification of spoilage microorganisms belongs to the basic challenges in food microbiology. The used identification methods, based on testing physiological and biochemical properties or analysing DNA mainly by PCR technique, aremore or less time and work demanding. Furthemore, the application of single methods usually differ for groups of microorganisms according to their phylogenetic relations. The MALDI-TOF MS method, working with protein profiles of microorganisms, providesa very rapid, easy to perform and reliable alternative, applicable to all microorganisms. In this study the identification of 420 bacteria and 50 yeasts at least isolated from dairy industry products, commodities and plants in Czech Republic in 2011-2013 was proved. The MALDI-TOF MS method with use of MALDI Biotyper 3.1 database and the appropriate biochemical tests and/or PCR based identification methods were applied. From Gram-positive bacteria the isolates of genera Bacillus, Lactoco

Název v anglickém jazyce

MALDI-TOF MS identification of spoilage microorganisms in dairy industry

Popis výsledku anglicky

The fast and reliable identification of spoilage microorganisms belongs to the basic challenges in food microbiology. The used identification methods, based on testing physiological and biochemical properties or analysing DNA mainly by PCR technique, aremore or less time and work demanding. Furthemore, the application of single methods usually differ for groups of microorganisms according to their phylogenetic relations. The MALDI-TOF MS method, working with protein profiles of microorganisms, providesa very rapid, easy to perform and reliable alternative, applicable to all microorganisms. In this study the identification of 420 bacteria and 50 yeasts at least isolated from dairy industry products, commodities and plants in Czech Republic in 2011-2013 was proved. The MALDI-TOF MS method with use of MALDI Biotyper 3.1 database and the appropriate biochemical tests and/or PCR based identification methods were applied. From Gram-positive bacteria the isolates of genera Bacillus, Lactoco

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1210300" target="_blank" >QJ1210300: Systémy jištění kvality a bezpečnosti mlékárenských výrobků vhodnými metodami aplikovatelnými v praxi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů