MALDI-TOF MS identification of spoilage and food-borne bacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F16%3AN0000034" target="_blank" >RIV/26722861:_____/16:N0000034 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/16:43903139

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

MALDI-TOF MS identification of spoilage and food-borne bacteria

Popis výsledku v původním jazyce

The fast and reliable identification and typing of spoilage and food-borne microorganisms belong to the basic challenges in food microbiology. Methods testing physiological and biochemical features or analysing DNA are more or less time and work demanding. Furthemore, bacteria phylogenetic groups differ in the applied specific method protocols. In comparison, MALDI-TOF MS analysis of bacterial protein profiles provides a very rapid alternative, easy to perform and widely applicable. In this study we concern two parts of interest. Firstly we identified 320 isolates of spoilage and food-borne bacteria from different sources by MALDI-TOF MS (using Bruker Autoflex Speed and Biotyper 3.1) and by biochemical or species-specific PCR methods, or 16S rRNA sequencing in discordant results. This comparison allowed to detect possible problematic species or genera as e.g. biochemically atypical strains of Raoultella ornithinolytica. The different clustering algorithms were designed and tested for the species discrimination within such some Pseudomonas, Enterobacter cloacae and Bacillus species group. Secondly we tested the influence of different diagnostic-selective media, the temperatures of cultivation and storage, and the sample preparation method on the robustness of MALDI-TOF MS identification of Enterobacteriaceae. The sample preparation method was found to be the most important factor for reliable identification in case of prolonged storage of cultures grown diagnostic-selective media.

Název v anglickém jazyce

MALDI-TOF MS identification of spoilage and food-borne bacteria

Popis výsledku anglicky

The fast and reliable identification and typing of spoilage and food-borne microorganisms belong to the basic challenges in food microbiology. Methods testing physiological and biochemical features or analysing DNA are more or less time and work demanding. Furthemore, bacteria phylogenetic groups differ in the applied specific method protocols. In comparison, MALDI-TOF MS analysis of bacterial protein profiles provides a very rapid alternative, easy to perform and widely applicable. In this study we concern two parts of interest. Firstly we identified 320 isolates of spoilage and food-borne bacteria from different sources by MALDI-TOF MS (using Bruker Autoflex Speed and Biotyper 3.1) and by biochemical or species-specific PCR methods, or 16S rRNA sequencing in discordant results. This comparison allowed to detect possible problematic species or genera as e.g. biochemically atypical strains of Raoultella ornithinolytica. The different clustering algorithms were designed and tested for the species discrimination within such some Pseudomonas, Enterobacter cloacae and Bacillus species group. Secondly we tested the influence of different diagnostic-selective media, the temperatures of cultivation and storage, and the sample preparation method on the robustness of MALDI-TOF MS identification of Enterobacteriaceae. The sample preparation method was found to be the most important factor for reliable identification in case of prolonged storage of cultures grown diagnostic-selective media.

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1210300" target="_blank" >QJ1210300: Systémy jištění kvality a bezpečnosti mlékárenských výrobků vhodnými metodami aplikovatelnými v praxi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů