CONTRIBUTION OF INDIRECT EFFECTS TO CLUSTERED DAMAGE IN DNA IRRADIATED WITH PROTONS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43899339" target="_blank" >RIV/60461373:22330/15:43899339 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389005:_____/15:00450488

Výsledek na webu

<a href="http://dx.doi.org/10.1093/rpd/ncv159" target="_blank" >http://dx.doi.org/10.1093/rpd/ncv159</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/rpd/ncv159" target="_blank" >10.1093/rpd/ncv159</a>

Jazyk výsledku

angličtina

Název v původním jazyce

CONTRIBUTION OF INDIRECT EFFECTS TO CLUSTERED DAMAGE IN DNA IRRADIATED WITH PROTONS

Popis výsledku v původním jazyce

Protons are the dominant particles both in galactic cosmic rays and in solar particle events and, furthermore, proton irradiation becomes increasingly used in tumour treatment. It is believed that complex DNA damage is the determining factor for the consequent cellular response to radiation. DNA plasmid pBR322 was irradiated at U120-M cyclotron with 30 MeV protons and treated with two Escherichia coli base excision repair enzymes. The yields of SSBs and DSBs were analysed using agarose gel electrophoresis. DNA has been irradiated in the presence of hydroxyl radical scavenger (coumarin-3-carboxylic acid) in order to distinguish between direct and indirect damage of the biological target. Pure scavenger solution was used as a probe for measurement of induced OH. radical yields. Experimental OH. radical yield kinetics was compared with predictions computed by two theoretical models-RADAMOL and Geant4-DNA. Both approaches use Geant4-DNA for description of physical stages of radiation action, and then each of them applies a distinct model for description of the pre-chemical and chemical stage.

Název v anglickém jazyce

CONTRIBUTION OF INDIRECT EFFECTS TO CLUSTERED DAMAGE IN DNA IRRADIATED WITH PROTONS

Popis výsledku anglicky

Protons are the dominant particles both in galactic cosmic rays and in solar particle events and, furthermore, proton irradiation becomes increasingly used in tumour treatment. It is believed that complex DNA damage is the determining factor for the consequent cellular response to radiation. DNA plasmid pBR322 was irradiated at U120-M cyclotron with 30 MeV protons and treated with two Escherichia coli base excision repair enzymes. The yields of SSBs and DSBs were analysed using agarose gel electrophoresis. DNA has been irradiated in the presence of hydroxyl radical scavenger (coumarin-3-carboxylic acid) in order to distinguish between direct and indirect damage of the biological target. Pure scavenger solution was used as a probe for measurement of induced OH. radical yields. Experimental OH. radical yield kinetics was compared with predictions computed by two theoretical models-RADAMOL and Geant4-DNA. Both approaches use Geant4-DNA for description of physical stages of radiation action, and then each of them applies a distinct model for description of the pre-chemical and chemical stage.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Radiation Protection Dosimetry

ISSN

0144-8420

e-ISSN

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Svazek periodika

166

Číslo periodika v rámci svazku

1-4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

44-48

Kód UT WoS článku

000361806600010

EID výsledku v databázi Scopus

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