Determination of Impurities in Bioproduced Succinic Acid
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43899340" target="_blank" >RIV/60461373:22330/15:43899340 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60461373:22310/15:43899340
Výsledek na webu
<a href="http://dx.doi.org/10.4172/2157-7064.1000264" target="_blank" >http://dx.doi.org/10.4172/2157-7064.1000264</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4172/2157-7064.1000264" target="_blank" >10.4172/2157-7064.1000264</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of Impurities in Bioproduced Succinic Acid
Popis výsledku v původním jazyce
At present, significant research resources are directed towards development of renewable products for replacing petrochemicals such as succinic acid. The critical component of this research is the identification of impurities which have a detrimental impact on further processing of succinic acid. We have adapted derivatization with gas chromatography - mass spectrometry to identify and quantify more than 120 impurities in several succinic acid samples. This study focused on petroleum based succinic acidas well as bio-based samples that use a modified E. coli strain for fermentation. To enable an accurate quantification of both the target product and common impurities, we evaluated the acetonitrile extraction efficiency as an alternative to direct derivatization, and then compared several derivatization agents for trimethylsilylation. A prior acetonitrile extraction was shown to be essential to detect impurities in trace concentrations. N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA)
Název v anglickém jazyce
Determination of Impurities in Bioproduced Succinic Acid
Popis výsledku anglicky
At present, significant research resources are directed towards development of renewable products for replacing petrochemicals such as succinic acid. The critical component of this research is the identification of impurities which have a detrimental impact on further processing of succinic acid. We have adapted derivatization with gas chromatography - mass spectrometry to identify and quantify more than 120 impurities in several succinic acid samples. This study focused on petroleum based succinic acidas well as bio-based samples that use a modified E. coli strain for fermentation. To enable an accurate quantification of both the target product and common impurities, we evaluated the acetonitrile extraction efficiency as an alternative to direct derivatization, and then compared several derivatization agents for trimethylsilylation. A prior acetonitrile extraction was shown to be essential to detect impurities in trace concentrations. N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA)
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EI - Biotechnologie a bionika
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Chromatography & Separation Techniques
ISSN
2157-7064
e-ISSN
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Svazek periodika
6
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
8
Strana od-do
1-8
Kód UT WoS článku
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EID výsledku v databázi Scopus
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