Continuous production of n-butanol by Clostridium pasteurianum DSM 525 using suspended and surface-immobilized cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43899521" target="_blank" >RIV/60461373:22330/15:43899521 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jbiotec.2015.10.008" target="_blank" >http://dx.doi.org/10.1016/j.jbiotec.2015.10.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jbiotec.2015.10.008" target="_blank" >10.1016/j.jbiotec.2015.10.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Continuous production of n-butanol by Clostridium pasteurianum DSM 525 using suspended and surface-immobilized cells

Popis výsledku v původním jazyce

For n-butanol production by Clostridium pasteurianum DSM 525, a modified reinforced Clostridium medium was used, where glucose was alternated with glycerol and two kinds of continuous fermentation were tested using suspended and surface immobilized cellson corn stover pieces. A steady state, with butanol productivity of 4.2 g/L h, was reached during the packed-bed continuous fermentation at a dilution rate of 0.44 hMINUS SIGN 1. The average n-butanol concentration, yield and the ratio of n-butanol/liquid by-products were 10.4 g/L, 33 % and 2.5, respectively. Unexpectedly, during continuous fermentation with suspended cells, at a dilution rate of 0.01 hMINUS SIGN 1, steady-state was not achieved and regular oscillations occurred in all measured variables, i.e. concentrations of glycerol, products and the number of cells stained with the fluorescent dyes carboxy fluorescein diacetate and propidium iodide. A possible explanation for oscillatory/steady-state behavior of suspended/surface-

Název v anglickém jazyce

Continuous production of n-butanol by Clostridium pasteurianum DSM 525 using suspended and surface-immobilized cells

Popis výsledku anglicky

For n-butanol production by Clostridium pasteurianum DSM 525, a modified reinforced Clostridium medium was used, where glucose was alternated with glycerol and two kinds of continuous fermentation were tested using suspended and surface immobilized cellson corn stover pieces. A steady state, with butanol productivity of 4.2 g/L h, was reached during the packed-bed continuous fermentation at a dilution rate of 0.44 hMINUS SIGN 1. The average n-butanol concentration, yield and the ratio of n-butanol/liquid by-products were 10.4 g/L, 33 % and 2.5, respectively. Unexpectedly, during continuous fermentation with suspended cells, at a dilution rate of 0.01 hMINUS SIGN 1, steady-state was not achieved and regular oscillations occurred in all measured variables, i.e. concentrations of glycerol, products and the number of cells stained with the fluorescent dyes carboxy fluorescein diacetate and propidium iodide. A possible explanation for oscillatory/steady-state behavior of suspended/surface-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/TE01020080" target="_blank" >TE01020080: Centrum kompetence pro výzkum biorafinací</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF BIOTECHNOLOGY

ISSN

0168-1656

e-ISSN

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Svazek periodika

216

Číslo periodika v rámci svazku

říjen

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

29-35

Kód UT WoS článku

000365769000004

EID výsledku v databázi Scopus

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