Application of matrix-assisted laser desorption and ionization time of flight mass spectrometry to the study of the proteinaceous binders in paint: blue paint composition in the series "The Life of Virgin" by Mons Cano (17th century) as a case study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43899577" target="_blank" >RIV/60461373:22330/15:43899577 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.impublications.com/content/abstract?code=E21_0019" target="_blank" >http://www.impublications.com/content/abstract?code=E21_0019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1255/ejms.1306" target="_blank" >10.1255/ejms.1306</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of matrix-assisted laser desorption and ionization time of flight mass spectrometry to the study of the proteinaceous binders in paint: blue paint composition in the series "The Life of Virgin" by Mons Cano (17th century) as a case study

Popis výsledku v původním jazyce

The identification of proteinaceous materials in paint constituents provides very valuable information regarding the techniques used by the painter and the most suitable procedures for conserving and restoring their works. Although the analysis of proteinaceous materials is nowadays a common task when dealing with works of art, the reliable detection and identification of protein traces is still complicated, particularly when very small samples can be taken that may contain a mixture of different organic materials (oils, waxes, resins, gums etc.). We therefore proposed a proteomic approach to investigate protein materials in paintings at trace levels in order to obtain a better understanding of the painter's technique. After trypsin digestion of the paint samples, mass spectra were obtained by matrix-assisted laser desorption and ionization time of flight mass spectrometry (MALDI-TOF-MS) and they were compared with the Mascot database and with theoretical digested proteins. This study

Název v anglickém jazyce

Application of matrix-assisted laser desorption and ionization time of flight mass spectrometry to the study of the proteinaceous binders in paint: blue paint composition in the series "The Life of Virgin" by Mons Cano (17th century) as a case study

Popis výsledku anglicky

The identification of proteinaceous materials in paint constituents provides very valuable information regarding the techniques used by the painter and the most suitable procedures for conserving and restoring their works. Although the analysis of proteinaceous materials is nowadays a common task when dealing with works of art, the reliable detection and identification of protein traces is still complicated, particularly when very small samples can be taken that may contain a mixture of different organic materials (oils, waxes, resins, gums etc.). We therefore proposed a proteomic approach to investigate protein materials in paintings at trace levels in order to obtain a better understanding of the painter's technique. After trypsin digestion of the paint samples, mass spectra were obtained by matrix-assisted laser desorption and ionization time of flight mass spectrometry (MALDI-TOF-MS) and they were compared with the Mascot database and with theoretical digested proteins. This study

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Mass Spectrometry

ISSN

1469-0667

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

19-26

Kód UT WoS článku

000355233800003

EID výsledku v databázi Scopus

—