PROTEOMICS TOOLS FOR THE CONTEMPORARY IDENTIFICATION OF PROTEINACEOUS BINDERS IN GILDED SAMPLES
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43899580" target="_blank" >RIV/60461373:22330/15:43899580 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.researchgate.net/publication/283802615_Proteomics_tools_for_the_contemporary_identification_of_proteinaceous_binders_in_gilded_samples" target="_blank" >https://www.researchgate.net/publication/283802615_Proteomics_tools_for_the_contemporary_identification_of_proteinaceous_binders_in_gilded_samples</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
PROTEOMICS TOOLS FOR THE CONTEMPORARY IDENTIFICATION OF PROTEINACEOUS BINDERS IN GILDED SAMPLES
Popis výsledku v původním jazyce
This paper deals with the characterization of several gilded and polychrome samples from the carved wood Portuguese heritage using a novel proteomics approach aimed to identify and map the presence of different proteinaceous binders. Eight samples originating from two altarpieces (Main altarpiece from Miranda de Douro Cathedral and Main altarpiece of the Cathedral in Funchal, Madeira) and three polychrome sculptures (St. Joseph from Aveiro Museum and two sculptures by Machado de Castro) were taken and analysed both as fragments/separated layers (e.g. ground layer separated from the gilded/polychrome layers) and cross-sections. The methodology proposed here makes use of proteomics techniques such as ELISA, MALDI-TOF-MS, nano-LC-ESI-Q-TOF and optical microscopy coupled with a fluorescent staining test (Sypro Ruby) on samples and cross-sections and is part of the analytical task of the Gilt-Teller project (www.gilt-teller.pt). ELISA, and both mass spectrometric techniques MALDI-TOF and na
Název v anglickém jazyce
PROTEOMICS TOOLS FOR THE CONTEMPORARY IDENTIFICATION OF PROTEINACEOUS BINDERS IN GILDED SAMPLES
Popis výsledku anglicky
This paper deals with the characterization of several gilded and polychrome samples from the carved wood Portuguese heritage using a novel proteomics approach aimed to identify and map the presence of different proteinaceous binders. Eight samples originating from two altarpieces (Main altarpiece from Miranda de Douro Cathedral and Main altarpiece of the Cathedral in Funchal, Madeira) and three polychrome sculptures (St. Joseph from Aveiro Museum and two sculptures by Machado de Castro) were taken and analysed both as fragments/separated layers (e.g. ground layer separated from the gilded/polychrome layers) and cross-sections. The methodology proposed here makes use of proteomics techniques such as ELISA, MALDI-TOF-MS, nano-LC-ESI-Q-TOF and optical microscopy coupled with a fluorescent staining test (Sypro Ruby) on samples and cross-sections and is part of the analytical task of the Gilt-Teller project (www.gilt-teller.pt). ELISA, and both mass spectrometric techniques MALDI-TOF and na
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
International journal of conservation science
ISSN
2067-533X
e-ISSN
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Svazek periodika
6
Číslo periodika v rámci svazku
SEP 2015
Stát vydavatele periodika
RO - Rumunsko
Počet stran výsledku
12
Strana od-do
507-518
Kód UT WoS článku
000364504100007
EID výsledku v databázi Scopus
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