Production of Palmitoleic and Linoleic Acid in Oleaginous and Nonoleaginous Yeast Biomass

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43900788" target="_blank" >RIV/60461373:22330/16:43900788 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/16:00462000

Výsledek na webu

<a href="http://dx.doi.org/10.1155/2016/7583684" target="_blank" >http://dx.doi.org/10.1155/2016/7583684</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2016/7583684" target="_blank" >10.1155/2016/7583684</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Production of Palmitoleic and Linoleic Acid in Oleaginous and Nonoleaginous Yeast Biomass

Popis výsledku v původním jazyce

We investigated the possibility of utilizing both oleaginous yeast species accumulating large amounts of lipids (Yarrowia lipolytica, Rhodotorula glutinis, Trichosporon cutaneum, and Candida sp.) and traditional biotechnological nonoleaginous ones (Kluyveromyces polysporus, Torulaspora delbrueckii, and Saccharomyces cerevisiae) as potential producers of dietetically important major fatty acids.Themain objective was to examine the cultivation conditions that would induce a high ratio of dietary fatty acids and biomass.Though genus-dependent, the type of nitrogen source had a higher influence on biomass yield than the C/N ratio.The nitrogen source leading to the highest lipid accumulation was potassium nitrate, followed by ammonium sulfate, which is an ideal nitrogen source supporting, in both oleaginous and nonoleaginous species, sufficient biomass growth with concomitantly increased lipid accumulation. All yeast strains displayed high (70-90%) content of unsaturated fatty acids in total cell lipids.The content of dietary fatty acids of interest, namely, palmitoleic acid and linoleic acid, reached in Kluyveromyces and Trichosporon strains over 50% of total fatty acids and the highest yield, over 280mg per g of dry cell weight of these fatty acids, was observed in Trichosporon with ammonium sulfate as nitrogen source at C/N ratio 70.

Název v anglickém jazyce

Production of Palmitoleic and Linoleic Acid in Oleaginous and Nonoleaginous Yeast Biomass

Popis výsledku anglicky

We investigated the possibility of utilizing both oleaginous yeast species accumulating large amounts of lipids (Yarrowia lipolytica, Rhodotorula glutinis, Trichosporon cutaneum, and Candida sp.) and traditional biotechnological nonoleaginous ones (Kluyveromyces polysporus, Torulaspora delbrueckii, and Saccharomyces cerevisiae) as potential producers of dietetically important major fatty acids.Themain objective was to examine the cultivation conditions that would induce a high ratio of dietary fatty acids and biomass.Though genus-dependent, the type of nitrogen source had a higher influence on biomass yield than the C/N ratio.The nitrogen source leading to the highest lipid accumulation was potassium nitrate, followed by ammonium sulfate, which is an ideal nitrogen source supporting, in both oleaginous and nonoleaginous species, sufficient biomass growth with concomitantly increased lipid accumulation. All yeast strains displayed high (70-90%) content of unsaturated fatty acids in total cell lipids.The content of dietary fatty acids of interest, namely, palmitoleic acid and linoleic acid, reached in Kluyveromyces and Trichosporon strains over 50% of total fatty acids and the highest yield, over 280mg per g of dry cell weight of these fatty acids, was observed in Trichosporon with ammonium sulfate as nitrogen source at C/N ratio 70.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/GA14-00227S" target="_blank" >GA14-00227S: Mikroorganizmy jako zdroje esenciálních mastných kyselin se zaměřením na extremofilní kmeny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Analytical Chemistry

ISSN

1687-8760

e-ISSN

—

Svazek periodika

2016

Číslo periodika v rámci svazku

únor

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1-9

Kód UT WoS článku

000372242000001

EID výsledku v databázi Scopus

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