Metabolomic Strategies Based on High-Resolution Mass Spectrometry as a Tool for Recognition of GMO (MON 89788 Variety) and Non-GMO Soybean: a Critical Assessment of Two Complementary Methods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F17%3A43913763" target="_blank" >RIV/60461373:22330/17:43913763 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22810/17:43913763 RIV/00027006:_____/17:00004243

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs12161-017-0929-8" target="_blank" >https://link.springer.com/article/10.1007%2Fs12161-017-0929-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12161-017-0929-8" target="_blank" >10.1007/s12161-017-0929-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Metabolomic Strategies Based on High-Resolution Mass Spectrometry as a Tool for Recognition of GMO (MON 89788 Variety) and Non-GMO Soybean: a Critical Assessment of Two Complementary Methods

Popis výsledku v původním jazyce

This study was focused on distinguishing of genetically modified organism (Monsanto 89788 variety) and conventional soybeans by employing high-resolution mass spectrometry (HRMS) techniques for non-target screening of sample extracts. Two hyphenated instrumental platforms represented by (i) ultrahigh-performance liquid chromatography (U-HPLC) coupled to quadrupole/time of flight and (ii) ambient mass spectrometry with direct analysis in real time (DART) ion source-coupled OrbitrapMS were used. The statistical processing of generated data (metabolomic fingerprints) was performed by multivariate data analysis; principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) showed that both employed techniques enabled correct classification of genetically modified and conventional soybeans. In addition, some phosphatidylcholines and sugars were identified as the most significant markers.

Název v anglickém jazyce

Metabolomic Strategies Based on High-Resolution Mass Spectrometry as a Tool for Recognition of GMO (MON 89788 Variety) and Non-GMO Soybean: a Critical Assessment of Two Complementary Methods

Popis výsledku anglicky

This study was focused on distinguishing of genetically modified organism (Monsanto 89788 variety) and conventional soybeans by employing high-resolution mass spectrometry (HRMS) techniques for non-target screening of sample extracts. Two hyphenated instrumental platforms represented by (i) ultrahigh-performance liquid chromatography (U-HPLC) coupled to quadrupole/time of flight and (ii) ambient mass spectrometry with direct analysis in real time (DART) ion source-coupled OrbitrapMS were used. The statistical processing of generated data (metabolomic fingerprints) was performed by multivariate data analysis; principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) showed that both employed techniques enabled correct classification of genetically modified and conventional soybeans. In addition, some phosphatidylcholines and sugars were identified as the most significant markers.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Analytical Methods

ISSN

1936-9751

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

3723-3737

Kód UT WoS článku

000412116000025

EID výsledku v databázi Scopus

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