Antibodies Modified by Gold Nanoparticles or Quantum Dots For Specific Detection of Sarcosine on The Membrane: Use in Rapid Diagnostics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F18%3A43915222" target="_blank" >RIV/60461373:22330/18:43915222 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/18:10392581

Výsledek na webu

<a href="https://www.nanocon.eu/files/uploads/01/NANOCON2017_Proceedings_content.pdf" target="_blank" >https://www.nanocon.eu/files/uploads/01/NANOCON2017_Proceedings_content.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Antibodies Modified by Gold Nanoparticles or Quantum Dots For Specific Detection of Sarcosine on The Membrane: Use in Rapid Diagnostics

Popis výsledku v původním jazyce

Paper tests are very cheap and fast diagnostic tools for self-testing in a wide range of health problems. Thus, the diagnostics of tumor development is of a great medical and socio-economic significance. Malignant prostate cancers are the most common malignancy in men. Procedures have been developed to diagnose this disease based on the monitoring of tumor marker molecules. Most commonly, PSA and fPSA are used. Although these tumor markers are significantly increased, the cancer at the biopsy is not always confirmed. Thus, the finding of other marker molecules is urgently needed. Recent studies have shown that changes in content of the amino acid sarcosine are associated with prostate tumors. Polyclonal chicken antibodies against sarcosine (AntiSar) were prepared. AntiSar antibodies were labeled with gold nanoparticles Au20 (size 20-30 nm, zeta potential - 38 mV) and CdTe QDs (emission 530 green QDs and 650 nm yellow QDs). Dot blot and flow lateral chromatography were used to develop a newly designed sarcosin detection procedure. The aggregation or fluorescence intensities were evaluated by newly designed software. Sarcosine was analyzed in a concentration range (0-150 μM) in PBS, arteficial urine and urine sample buffer. The calibration curves obtained in the concentration range (0-12 μM) were strictly linear (R2 = 0.99) with a relative error of 10%. Using the proposed methods sarcosine concentrations as low as 250-500 nM were analyzed. Moreover, the results obtained show a higher sensitivity (by 20-40%) of sarcosine detection using QDs in comparison with using gold nanoparticles.

Název v anglickém jazyce

Antibodies Modified by Gold Nanoparticles or Quantum Dots For Specific Detection of Sarcosine on The Membrane: Use in Rapid Diagnostics

Popis výsledku anglicky

Paper tests are very cheap and fast diagnostic tools for self-testing in a wide range of health problems. Thus, the diagnostics of tumor development is of a great medical and socio-economic significance. Malignant prostate cancers are the most common malignancy in men. Procedures have been developed to diagnose this disease based on the monitoring of tumor marker molecules. Most commonly, PSA and fPSA are used. Although these tumor markers are significantly increased, the cancer at the biopsy is not always confirmed. Thus, the finding of other marker molecules is urgently needed. Recent studies have shown that changes in content of the amino acid sarcosine are associated with prostate tumors. Polyclonal chicken antibodies against sarcosine (AntiSar) were prepared. AntiSar antibodies were labeled with gold nanoparticles Au20 (size 20-30 nm, zeta potential - 38 mV) and CdTe QDs (emission 530 green QDs and 650 nm yellow QDs). Dot blot and flow lateral chromatography were used to develop a newly designed sarcosin detection procedure. The aggregation or fluorescence intensities were evaluated by newly designed software. Sarcosine was analyzed in a concentration range (0-150 μM) in PBS, arteficial urine and urine sample buffer. The calibration curves obtained in the concentration range (0-12 μM) were strictly linear (R2 = 0.99) with a relative error of 10%. Using the proposed methods sarcosine concentrations as low as 250-500 nM were analyzed. Moreover, the results obtained show a higher sensitivity (by 20-40%) of sarcosine detection using QDs in comparison with using gold nanoparticles.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Nanocon 2017

ISBN

978-80-87294-81-9

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

6

Strana od-do

517-523

Název nakladatele

Tanger s.r.o.

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

18. 10. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000452823300085