Selection of Bacterial Strain for Lactic Acid Production from Straw Hydrolysate

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43918838" target="_blank" >RIV/60461373:22330/19:43918838 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Selection of Bacterial Strain for Lactic Acid Production from Straw Hydrolysate

Popis výsledku v původním jazyce

The objective of our project was to design and optimize the process of microbial production of lactic acid using an alternative, inexpensive substrate with achievement of lactic acid high yield. Fermentations were performed using a low-cost source of carbon, wheat straw hydrolysate. Identification tests of two strains of the genus Lactobacillus obtained from our laboratory were first performed to confirm or disprove their taxonomic classification. Firstly API 50 CHL test was performed. This test includes 50 biochemical tests, where fermentation of 49 saccharides in strips is assessed. For more accurate identification, MALDI-TOF was performed, which resulted in re-identification of the two strains as Lactobacillus casei 198 and Lactobacillus plantarum LHR14. Thereafter, MRS media screening tests were performed with these two strains, where, glucose, sucrose and xylose were tested as carbon sources. Further, inhibitors present in the straw hydrolysate such as coumaric acid, ferulic acid and syringaldehyde in concentrations ranging from 0.2 to 2.5 g.l-1 and potassium hydrogen phosphate at a concentration from 15 to 35 g.l-1 were added to the medium and growth and lactic acid production were tested.

Název v anglickém jazyce

Selection of Bacterial Strain for Lactic Acid Production from Straw Hydrolysate

Popis výsledku anglicky

The objective of our project was to design and optimize the process of microbial production of lactic acid using an alternative, inexpensive substrate with achievement of lactic acid high yield. Fermentations were performed using a low-cost source of carbon, wheat straw hydrolysate. Identification tests of two strains of the genus Lactobacillus obtained from our laboratory were first performed to confirm or disprove their taxonomic classification. Firstly API 50 CHL test was performed. This test includes 50 biochemical tests, where fermentation of 49 saccharides in strips is assessed. For more accurate identification, MALDI-TOF was performed, which resulted in re-identification of the two strains as Lactobacillus casei 198 and Lactobacillus plantarum LHR14. Thereafter, MRS media screening tests were performed with these two strains, where, glucose, sucrose and xylose were tested as carbon sources. Further, inhibitors present in the straw hydrolysate such as coumaric acid, ferulic acid and syringaldehyde in concentrations ranging from 0.2 to 2.5 g.l-1 and potassium hydrogen phosphate at a concentration from 15 to 35 g.l-1 were added to the medium and growth and lactic acid production were tested.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

20901 - Industrial biotechnology

Projekt

<a href="/cs/project/LTACH17006" target="_blank" >LTACH17006: Výroba biobutanolu a kyseliny jantarové/mléčné z lignocelulózové biomasy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 7th International Conference on Chemical Technology

ISBN

978-80-88307-00-6

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

26-31

Název nakladatele

Czech Society of Industrial Chemistry

Místo vydání

Prague

Místo konání akce

Mikulov

Datum konání akce

15. 4. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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