Development of method for analysis of phytocannabinoids in plasma of laboratory animals: toxicokinetic study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919211" target="_blank" >RIV/60461373:22330/19:43919211 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of method for analysis of phytocannabinoids in plasma of laboratory animals: toxicokinetic study

Popis výsledku v původním jazyce

Although positive effects of phytocannabinoids on the human body, their fate in the organism has not been thoroughly described. The aim of the study was developed, optimize and validate based on ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC MS/MS) for the target analysis of 17 phytocannabinoids and three Δ9-THC metabolites in rat blood plasma. After addition acetonitrile to plasma sample and removing of precipitate by centrifugation, the samples were ready for analysis. The recoveries of phytocannabinoids ranged from 82-to111% and in case of THC metabolites from 113 to 121%. The repeat abilities of measurements expressed as RSD were in range 3% - 9%. The linear range was from 0.5 ng / ml to 250 ng / ml of plasma. The method was applied for analysis of 219 blood plasma samples of rats that were given pure cannabidiol (CBD) in the form of six different carriers’ formulations to determine the bioavailability of CBD. The concentration profile of CBD after its administration was recorded. The lowest availability was observed for pure CBD. Using high-resolution mass spectrometry (HRMS), further screening of other phytocannabinoids and their metabolites was performed. Interestingly, number of phytocannabinoids and various phytocannabinoids metabolites was detected in rats´ plasma although pure CBD was administered.

Název v anglickém jazyce

Development of method for analysis of phytocannabinoids in plasma of laboratory animals: toxicokinetic study

Popis výsledku anglicky

Although positive effects of phytocannabinoids on the human body, their fate in the organism has not been thoroughly described. The aim of the study was developed, optimize and validate based on ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC MS/MS) for the target analysis of 17 phytocannabinoids and three Δ9-THC metabolites in rat blood plasma. After addition acetonitrile to plasma sample and removing of precipitate by centrifugation, the samples were ready for analysis. The recoveries of phytocannabinoids ranged from 82-to111% and in case of THC metabolites from 113 to 121%. The repeat abilities of measurements expressed as RSD were in range 3% - 9%. The linear range was from 0.5 ng / ml to 250 ng / ml of plasma. The method was applied for analysis of 219 blood plasma samples of rats that were given pure cannabidiol (CBD) in the form of six different carriers’ formulations to determine the bioavailability of CBD. The concentration profile of CBD after its administration was recorded. The lowest availability was observed for pure CBD. Using high-resolution mass spectrometry (HRMS), further screening of other phytocannabinoids and their metabolites was performed. Interestingly, number of phytocannabinoids and various phytocannabinoids metabolites was detected in rats´ plasma although pure CBD was administered.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/TN01000048" target="_blank" >TN01000048: Biorafinace jako oběhové technologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů