Development of UHPLC-MS/MS method for studies of phytocannabinoids fate in experimental organisms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925469" target="_blank" >RIV/60461373:22330/22:43925469 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of UHPLC-MS/MS method for studies of phytocannabinoids fate in experimental organisms

Popis výsledku v původním jazyce

Phytocannabinoids are unique biologically active metabolites of hemp (Cannabis sativa L.) with a wide therapeutic potential. Besides psychotropic Δ9-tetrahydrocannabinol (Δ9-THC), exponentially growing attention has been paid to its non-psychotropic isomer, cannabidiol (CBD). Phytocannabinoids are used to alleviate the symptoms of various diseases, in the form of isolated substances or complex hemp extracts, alternatively or in the form of dietary supplements such as CBD oils. Needed to emphasize that Cannabis plants contain dozens of other phytocannabinoids (large range of isomeric structures) and non-cannabinoid biologically active substances (phenols, terpenoids, etc.), thus the detailed analysis of obtained extracts becomes an analytical challenge. Due to their lipophilic nature, phytocannabinoids are mainly absorbed by the tissues of the heart, lungs, liver, and brain, while their distribution from the blood to the tissues is relatively fast. To understand dose-effect relationships, investigation of parent compound distribution within the tissue of exposed organisms and monitoring of their further transformations is of high concern. In the presented study focused on the transfer of phytocannabinoids into the brain tissue of 200 exposed animals administered a CBD preparation encapsulated with various carriers (oil with free CBD as a reference), an analytical method was developed, optimized, and validated for the target analysis of 18 phytocannabinoids, 3 Δ9-THC metabolites, and 2 CBD metabolites using reversed phase ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). The recoveries of phytocannabinoids ranged from 61 % to 121 %. The repeatabilities of measurements expressed as RSD were within a range of 1 % to 20 %. The linear range of the analytical method for individual phytocannabinoids was 1 - 250 ng/ml in extract and the limit of quantification (LOQ) was determined between 1 - 20 ng/ml in the extract.

Název v anglickém jazyce

Development of UHPLC-MS/MS method for studies of phytocannabinoids fate in experimental organisms

Popis výsledku anglicky

Phytocannabinoids are unique biologically active metabolites of hemp (Cannabis sativa L.) with a wide therapeutic potential. Besides psychotropic Δ9-tetrahydrocannabinol (Δ9-THC), exponentially growing attention has been paid to its non-psychotropic isomer, cannabidiol (CBD). Phytocannabinoids are used to alleviate the symptoms of various diseases, in the form of isolated substances or complex hemp extracts, alternatively or in the form of dietary supplements such as CBD oils. Needed to emphasize that Cannabis plants contain dozens of other phytocannabinoids (large range of isomeric structures) and non-cannabinoid biologically active substances (phenols, terpenoids, etc.), thus the detailed analysis of obtained extracts becomes an analytical challenge. Due to their lipophilic nature, phytocannabinoids are mainly absorbed by the tissues of the heart, lungs, liver, and brain, while their distribution from the blood to the tissues is relatively fast. To understand dose-effect relationships, investigation of parent compound distribution within the tissue of exposed organisms and monitoring of their further transformations is of high concern. In the presented study focused on the transfer of phytocannabinoids into the brain tissue of 200 exposed animals administered a CBD preparation encapsulated with various carriers (oil with free CBD as a reference), an analytical method was developed, optimized, and validated for the target analysis of 18 phytocannabinoids, 3 Δ9-THC metabolites, and 2 CBD metabolites using reversed phase ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). The recoveries of phytocannabinoids ranged from 61 % to 121 %. The repeatabilities of measurements expressed as RSD were within a range of 1 % to 20 %. The linear range of the analytical method for individual phytocannabinoids was 1 - 250 ng/ml in extract and the limit of quantification (LOQ) was determined between 1 - 20 ng/ml in the extract.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LM2018100" target="_blank" >LM2018100: Infrastruktura pro propagaci metrologie v potravinářství a výživě v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů