Fast analysis of phytocannabinoids in plant matrices and products thereof by SFC-HRMS/MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925458" target="_blank" >RIV/60461373:22330/22:43925458 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fast analysis of phytocannabinoids in plant matrices and products thereof by SFC-HRMS/MS

Popis výsledku v původním jazyce

The interest in hemp products such as seeds and cold pressed oil is rapidly growing. Although hemp seeds themselves do not naturally contain phytocannabinoids including psychotropic delta-9-tetrahydrocannabinol (Δ9-THC) or its precursor delta-9- tetrahydrocannabinolic acid (Δ9-THCA), contamination may occur during harvest and/or processing. To prevent consumers´ health risks, the presence of this phytocannabinoid in hemp-based foods should be monitored. Until recently, no EU regulation (maximum limits) was available, nevertheless, at present, the determined concentration of total THC (sum of free Δ9-THC and that bound in Δ9-THCA) can be assessed against ´new´ EU limits: specifically, 7.5 mg/kg set for hemp oil and 3.0 mg/kg for dry foods (flour, proteins, seeds, snacks). In response to the urgently needed effective control tool, new method employing supercritical fluid chromatography coupled to high resolution mass spectrometry (SFC-HRMS) was developed and validated for altogether 19 phytocannabinoids. The recoveries ranged from 71 % to 105 %. The repeatability, expressed as relative standard deviation (RSD), ranged from 5 % to 15 %. The linear range of the analytical method for individual phytocannabinoids was from 0,05-1,0 mg/kg (seeds) and 0.25-5.0 mg/kg (oil). The limit of quantification (LOQ) ranged from 0.05-0.25 mg/kg (seeds) and 0.25-1.25 mg/kg (oil). Furthermore, the method was used for the analysis of 10 hemp seed samples and 10 samples of hemp oil collected at the market. The total phytocannabinoid content of the seeds ranged from 4 - 49 mg/kg and 2 seed samples exceeded the legal limit for &apos;total&apos; THC by 30 and 250 %. For hemp oils, the phytocannabinoid content ranged from 13 - 1400 mg/kg, and 3 samples exceeded the limit for &apos;total&apos; THC by 60, 610, and 620 %. In one oil sample, all analytes determine were below the LOQ.

Název v anglickém jazyce

Fast analysis of phytocannabinoids in plant matrices and products thereof by SFC-HRMS/MS

Popis výsledku anglicky

The interest in hemp products such as seeds and cold pressed oil is rapidly growing. Although hemp seeds themselves do not naturally contain phytocannabinoids including psychotropic delta-9-tetrahydrocannabinol (Δ9-THC) or its precursor delta-9- tetrahydrocannabinolic acid (Δ9-THCA), contamination may occur during harvest and/or processing. To prevent consumers´ health risks, the presence of this phytocannabinoid in hemp-based foods should be monitored. Until recently, no EU regulation (maximum limits) was available, nevertheless, at present, the determined concentration of total THC (sum of free Δ9-THC and that bound in Δ9-THCA) can be assessed against ´new´ EU limits: specifically, 7.5 mg/kg set for hemp oil and 3.0 mg/kg for dry foods (flour, proteins, seeds, snacks). In response to the urgently needed effective control tool, new method employing supercritical fluid chromatography coupled to high resolution mass spectrometry (SFC-HRMS) was developed and validated for altogether 19 phytocannabinoids. The recoveries ranged from 71 % to 105 %. The repeatability, expressed as relative standard deviation (RSD), ranged from 5 % to 15 %. The linear range of the analytical method for individual phytocannabinoids was from 0,05-1,0 mg/kg (seeds) and 0.25-5.0 mg/kg (oil). The limit of quantification (LOQ) ranged from 0.05-0.25 mg/kg (seeds) and 0.25-1.25 mg/kg (oil). Furthermore, the method was used for the analysis of 10 hemp seed samples and 10 samples of hemp oil collected at the market. The total phytocannabinoid content of the seeds ranged from 4 - 49 mg/kg and 2 seed samples exceeded the legal limit for &apos;total&apos; THC by 30 and 250 %. For hemp oils, the phytocannabinoid content ranged from 13 - 1400 mg/kg, and 3 samples exceeded the limit for &apos;total&apos; THC by 60, 610, and 620 %. In one oil sample, all analytes determine were below the LOQ.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LM2018100" target="_blank" >LM2018100: Infrastruktura pro propagaci metrologie v potravinářství a výživě v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů