PHYTOCANNABINOID PROFILES IN HEMP SEEDS AND PRODUCTS THEREOF

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F23%3A43928065" target="_blank" >RIV/60461373:22330/23:43928065 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

PHYTOCANNABINOID PROFILES IN HEMP SEEDS AND PRODUCTS THEREOF

Popis výsledku v původním jazyce

Cannabis sativa L. (hemp) is a plant that contains unique secondary metabolites called phytocannabinoids. Currently, this plant is widely used in the food industry, with hemp seeds and derived products such as hemp oil and flour being particularly popular. The seeds themselves contains antioxidants and polyunsaturated fatty acids but they do not contain any phytocannabinoids unless they come into contact with resin found in the glandular trichomes of female flowers. While there are many phytocannabinoids, those belonging to the tetrahydrocannabinol group are regulated by law due to their psychoactive effects. In relation to the need for monitoring the content of psychoactive substances, a maximum limit has been set for the sum of delta-9-tetrahydrocannabinol (Δ9-THC) and its acidic precursor delta-9-tetrahydrocannabinolic acid (Δ9-THCA). In 2022, the European Commission approved an amendment to Commission Regulation (EU) No 1881/2006, which sets maximum limits for certain contaminants in food, including Δ9-THC. The limit is set at 3 mg/kg for hemp seeds and products derived from hemp seeds, and 7.5 mg/kg for hemp oil. This study focuses on the quantitative determination of 42 phytocannabinoids in hemp seeds and oils or flour. The obtained results of Δ9-THC content were also subsequently compared with the Acute Reference Dose (ARfD) established by EFSA. The analysis was performed using ultra-high performance liquid chromatography coupled with high resolution mass spectrometry (U-HPLC-HRMS). The results revealed diverse profiles of phytocannabinoids in hemp seeds and their products. The seeds contained low concentrations of target analytes, with non-psychoactive components and predominantly acidic forms of phytocannabinoids, such as cannabidiolic acid (CBDA) or cannabidivarinic acid (CBDVA), being predominant. Oils and flours exhibited varying ratios of phytocannabinoids. The highest finding of total phytocannabinoid content in hemp oils exceeded 1400 mg/kg. Such a high finding was observed in only 2 samples, while the remaining samples did not exceed the level of total phytocannabinoid content of 100 mg/kg. In each tested product group, there was a product that would exceed the ARfD set for Δ9-THC when consumed in the recommended amount. These findings can be used for the assessment and quality control of these products, as well as for further research and development in the field of hemp products with therapeutic potential.

Název v anglickém jazyce

PHYTOCANNABINOID PROFILES IN HEMP SEEDS AND PRODUCTS THEREOF

Popis výsledku anglicky

Cannabis sativa L. (hemp) is a plant that contains unique secondary metabolites called phytocannabinoids. Currently, this plant is widely used in the food industry, with hemp seeds and derived products such as hemp oil and flour being particularly popular. The seeds themselves contains antioxidants and polyunsaturated fatty acids but they do not contain any phytocannabinoids unless they come into contact with resin found in the glandular trichomes of female flowers. While there are many phytocannabinoids, those belonging to the tetrahydrocannabinol group are regulated by law due to their psychoactive effects. In relation to the need for monitoring the content of psychoactive substances, a maximum limit has been set for the sum of delta-9-tetrahydrocannabinol (Δ9-THC) and its acidic precursor delta-9-tetrahydrocannabinolic acid (Δ9-THCA). In 2022, the European Commission approved an amendment to Commission Regulation (EU) No 1881/2006, which sets maximum limits for certain contaminants in food, including Δ9-THC. The limit is set at 3 mg/kg for hemp seeds and products derived from hemp seeds, and 7.5 mg/kg for hemp oil. This study focuses on the quantitative determination of 42 phytocannabinoids in hemp seeds and oils or flour. The obtained results of Δ9-THC content were also subsequently compared with the Acute Reference Dose (ARfD) established by EFSA. The analysis was performed using ultra-high performance liquid chromatography coupled with high resolution mass spectrometry (U-HPLC-HRMS). The results revealed diverse profiles of phytocannabinoids in hemp seeds and their products. The seeds contained low concentrations of target analytes, with non-psychoactive components and predominantly acidic forms of phytocannabinoids, such as cannabidiolic acid (CBDA) or cannabidivarinic acid (CBDVA), being predominant. Oils and flours exhibited varying ratios of phytocannabinoids. The highest finding of total phytocannabinoid content in hemp oils exceeded 1400 mg/kg. Such a high finding was observed in only 2 samples, while the remaining samples did not exceed the level of total phytocannabinoid content of 100 mg/kg. In each tested product group, there was a product that would exceed the ARfD set for Δ9-THC when consumed in the recommended amount. These findings can be used for the assessment and quality control of these products, as well as for further research and development in the field of hemp products with therapeutic potential.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40500 - Other agricultural sciences

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů