Determination of seventeen phytocannabinoids in various matrices by UHPLC-HRMS/MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43920883" target="_blank" >RIV/60461373:22330/20:43920883 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.chromatographyonline.com/view/determination-seventeen-phytocannabinoids-various-matrices-uhplc-hrmsms" target="_blank" >https://www.chromatographyonline.com/view/determination-seventeen-phytocannabinoids-various-matrices-uhplc-hrmsms</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of seventeen phytocannabinoids in various matrices by UHPLC-HRMS/MS

Popis výsledku v původním jazyce

A simple ultrahigh-performance liquid chromatography high resolution tandem mass spectrometry (UHPLC− HRMS/MS) method to determine seventeen phytocannabinoids was developed and validated for Cannabis plants, resins and their extracts, and oils. The analysis was challenging because of the complexity of matrices, the large differences in the concentration of phytocannabinoids and their pattern in various cultivars, and the structural similarity of these analytes. Method performance characteristics were obtained using internal reference materials. The analytes were isolated from dry hemp by repeated extraction using ethanol with recoveries in the range of 95–100%. For the resinous matrices and oils, samples were directly dissolved in ethanol. Repeatability was 2–12% for all analyte–matrix combinations. Limits of quantification (LOQs) were within the range of 0.5–1.0 mg/kg for dry hemp, 2.5–5.0 mg/kg for resins, and 0.25–0.50 mg/kg for oils. As a result of high matrix dilution, calibration standards in net solvent could be used for phytocannabinoids quantification because matrix effects were almost eliminated. Nevertheless, to ensure the trueness of generated data regardless of the matrix tested, internal standards of delta‑9‑tetrahydrocannabinol-D3 (Δ9-THC-D3), cannabidiol-D3 (CBD-D3) and cannabinol-D3 (CBN-D3) were also used.

Název v anglickém jazyce

Determination of seventeen phytocannabinoids in various matrices by UHPLC-HRMS/MS

Popis výsledku anglicky

A simple ultrahigh-performance liquid chromatography high resolution tandem mass spectrometry (UHPLC− HRMS/MS) method to determine seventeen phytocannabinoids was developed and validated for Cannabis plants, resins and their extracts, and oils. The analysis was challenging because of the complexity of matrices, the large differences in the concentration of phytocannabinoids and their pattern in various cultivars, and the structural similarity of these analytes. Method performance characteristics were obtained using internal reference materials. The analytes were isolated from dry hemp by repeated extraction using ethanol with recoveries in the range of 95–100%. For the resinous matrices and oils, samples were directly dissolved in ethanol. Repeatability was 2–12% for all analyte–matrix combinations. Limits of quantification (LOQs) were within the range of 0.5–1.0 mg/kg for dry hemp, 2.5–5.0 mg/kg for resins, and 0.25–0.50 mg/kg for oils. As a result of high matrix dilution, calibration standards in net solvent could be used for phytocannabinoids quantification because matrix effects were almost eliminated. Nevertheless, to ensure the trueness of generated data regardless of the matrix tested, internal standards of delta‑9‑tetrahydrocannabinol-D3 (Δ9-THC-D3), cannabidiol-D3 (CBD-D3) and cannabinol-D3 (CBN-D3) were also used.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

LC GC Europe

ISSN

1471-6577

e-ISSN

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Svazek periodika

33

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

8-16

Kód UT WoS článku

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EID výsledku v databázi Scopus

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