Analysis of phytocannabinoids in plant oils: benefits provided by supercritical fluid chromatography (SFC) coupled to high resolution mass spectrometry (HRMS)

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of phytocannabinoids in plant oils: benefits provided by supercritical fluid chromatography (SFC) coupled to high resolution mass spectrometry (HRMS)

Popis výsledku v původním jazyce

A fast supercritical fluid chromatography – high resolution mass spectrometry based method (SFCHRMS/MS) for the determination of 17 phytocannabinoids in plant oils has been developed and validated. Analysis of phytocannabinoids in oils is a challenging task not only due to a difficult matrix, but also because of a structural similarity and high concentration variability of the target analytes. Prior the instrumental analysis, samples were only dissolved in hexane and then separated on a normal phase Acquity UPC2 Torus DIOL column using 5 mM ammonium formate and 0,1% formic acid in methanol:water (95:5, v/v) as mobile phases modifier; mass spectrometer with Q-TOF mass analyzer was used as the detector. The repeatabilities of measurements were in the range 4 – 14 % and the limits of quantitation ranged from 0.25 to 1.0 mg/kg for all analytes/oil matrix combinations. These results were comparable to those obtained by the commonly used reverse phase UHPLCHRMS/MS based methods, nevertheless, thanks to a low lipids retention under SFC conditions used, the sample analysis time was significantly reduced. Great attention was also paid to the evaluation of matrix effects as significant differences were found among plant oils (rapeseed, olive, hemp and linseed oils were analyzed). To compensate the matrix effects, deuterated internal standards of cannabidiol (CBD), Δ9-tetrahydrocannabinol (Δ9-THC) and cannabinol (CBN) were used.

Název v anglickém jazyce

Analysis of phytocannabinoids in plant oils: benefits provided by supercritical fluid chromatography (SFC) coupled to high resolution mass spectrometry (HRMS)

Popis výsledku anglicky

A fast supercritical fluid chromatography – high resolution mass spectrometry based method (SFCHRMS/MS) for the determination of 17 phytocannabinoids in plant oils has been developed and validated. Analysis of phytocannabinoids in oils is a challenging task not only due to a difficult matrix, but also because of a structural similarity and high concentration variability of the target analytes. Prior the instrumental analysis, samples were only dissolved in hexane and then separated on a normal phase Acquity UPC2 Torus DIOL column using 5 mM ammonium formate and 0,1% formic acid in methanol:water (95:5, v/v) as mobile phases modifier; mass spectrometer with Q-TOF mass analyzer was used as the detector. The repeatabilities of measurements were in the range 4 – 14 % and the limits of quantitation ranged from 0.25 to 1.0 mg/kg for all analytes/oil matrix combinations. These results were comparable to those obtained by the commonly used reverse phase UHPLCHRMS/MS based methods, nevertheless, thanks to a low lipids retention under SFC conditions used, the sample analysis time was significantly reduced. Great attention was also paid to the evaluation of matrix effects as significant differences were found among plant oils (rapeseed, olive, hemp and linseed oils were analyzed). To compensate the matrix effects, deuterated internal standards of cannabidiol (CBD), Δ9-tetrahydrocannabinol (Δ9-THC) and cannabinol (CBN) were used.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů