Monitoring of phytocannabinoids in hemp seeds and their products

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925475" target="_blank" >RIV/60461373:22330/22:43925475 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of phytocannabinoids in hemp seeds and their products

Popis výsledku v původním jazyce

In the first phase of the experimental part, the content of 17 phytocannabinoids was determined in 10 samples of hemp seeds and 10 samples of hemp oil available on the Czech market. Hemp seeds were homogenized, weighed, and an appropriate amount of dichloromethane-methanol was added. The suspension was shaken, filtered, and collected for analysis. A mixture of deuterated internal standards was added to the sample taken in this way. Quantification was performed using an external matrix calibration where the matrix was without phytocannabinoids. In the case of hemp oils, only the weighed sample was dissolved in ethanol. The sample prepared this way was also taken for analysis with the addition of a mixture of internal deuterated standards. Quantification was performed using an external solvent calibration. The analysis was carried out using the technique of reverse-phase ultra-high performance liquid chromatography coupled with tandem high-resolution mass spectrometry (UHPLC-HRMS/MS). Electrospray (ESI) operated both in positive and negative mode and was used for ionization. A quadrupole-orbitrap was used as a mass analyzer. The identification of the analyzed analytes was carried out on the basis of matching the retention times with the retention times of the analytical standards, then using the exact mass m/z of the ions of the analytes and the analytes isotopic.

Název v anglickém jazyce

Monitoring of phytocannabinoids in hemp seeds and their products

Popis výsledku anglicky

In the first phase of the experimental part, the content of 17 phytocannabinoids was determined in 10 samples of hemp seeds and 10 samples of hemp oil available on the Czech market. Hemp seeds were homogenized, weighed, and an appropriate amount of dichloromethane-methanol was added. The suspension was shaken, filtered, and collected for analysis. A mixture of deuterated internal standards was added to the sample taken in this way. Quantification was performed using an external matrix calibration where the matrix was without phytocannabinoids. In the case of hemp oils, only the weighed sample was dissolved in ethanol. The sample prepared this way was also taken for analysis with the addition of a mixture of internal deuterated standards. Quantification was performed using an external solvent calibration. The analysis was carried out using the technique of reverse-phase ultra-high performance liquid chromatography coupled with tandem high-resolution mass spectrometry (UHPLC-HRMS/MS). Electrospray (ESI) operated both in positive and negative mode and was used for ionization. A quadrupole-orbitrap was used as a mass analyzer. The identification of the analyzed analytes was carried out on the basis of matching the retention times with the retention times of the analytical standards, then using the exact mass m/z of the ions of the analytes and the analytes isotopic.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10700 - Other natural sciences

Projekt

<a href="/cs/project/LM2018100" target="_blank" >LM2018100: Infrastruktura pro propagaci metrologie v potravinářství a výživě v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů