Screening of phytocannabinoids and other bioactive metabolites in cannabis based medicinal products and food supplements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919212" target="_blank" >RIV/60461373:22330/19:43919212 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Screening of phytocannabinoids and other bioactive metabolites in cannabis based medicinal products and food supplements

Popis výsledku v původním jazyce

Cannabis plants are a key source of unique biologically active compounds - phytocannabinoids. Apart of psychotropic activity of most known phytocannabinoid, delta-9-tetrahydrokanabinol, these substances may exhibit a number of positive effects (analgesic, antioxidant, neuroprotective) on a human organism. However, worth to emphasize, that the overall biological effect of cannabis based products is associated with the presence of many other substances (entourage effect), such as terpenes, flavonoids, or phenolics; hundreds of them have been identified so far. Using in-house constructed spectral library, targeted screening of 227 minor phytocannabinoids and 151 other bioactive substances was performed on 52 samples, including extracts from various cultivars of technical and medicinal cannabis, cannabis-based food supplements, medicinal, and cosmetic products. Ultra-performance liquid chromatography coupled with high-resolution tandem mass spectrometry (UHPLC-HRMS) was in the first step optimized to improve the chromatographic separation of analytes and then applied to the sample analysis. In analyzed samples, in total 440 phytocannabinoids and 186 other biologically active substances were detected and tentatively identified. While the targeted metabolites profiles in CBD oils made from hemp extract were very rich, only limited number of screened compounds was detected in products containing hemp seeds (hemp oils, hemp oil-containing cosmetics, hemp protein). Hemp teas showed a great similarity with technical cultivars of cannabis, while profiles of medicinal cultivars were richer and more intense compared to technical ones. Based on the comparison of experimental and theoretical fragmentation HRMS spectra obtained in the analysis of cannabis cultivars a spectral database was created as a tool for confirmation of substances. So far, 66 fragmentation spectra of phytocannabinoids and 12 fragmentation spectra of other biologically active substances have been obtained.

Název v anglickém jazyce

Screening of phytocannabinoids and other bioactive metabolites in cannabis based medicinal products and food supplements

Popis výsledku anglicky

Cannabis plants are a key source of unique biologically active compounds - phytocannabinoids. Apart of psychotropic activity of most known phytocannabinoid, delta-9-tetrahydrokanabinol, these substances may exhibit a number of positive effects (analgesic, antioxidant, neuroprotective) on a human organism. However, worth to emphasize, that the overall biological effect of cannabis based products is associated with the presence of many other substances (entourage effect), such as terpenes, flavonoids, or phenolics; hundreds of them have been identified so far. Using in-house constructed spectral library, targeted screening of 227 minor phytocannabinoids and 151 other bioactive substances was performed on 52 samples, including extracts from various cultivars of technical and medicinal cannabis, cannabis-based food supplements, medicinal, and cosmetic products. Ultra-performance liquid chromatography coupled with high-resolution tandem mass spectrometry (UHPLC-HRMS) was in the first step optimized to improve the chromatographic separation of analytes and then applied to the sample analysis. In analyzed samples, in total 440 phytocannabinoids and 186 other biologically active substances were detected and tentatively identified. While the targeted metabolites profiles in CBD oils made from hemp extract were very rich, only limited number of screened compounds was detected in products containing hemp seeds (hemp oils, hemp oil-containing cosmetics, hemp protein). Hemp teas showed a great similarity with technical cultivars of cannabis, while profiles of medicinal cultivars were richer and more intense compared to technical ones. Based on the comparison of experimental and theoretical fragmentation HRMS spectra obtained in the analysis of cannabis cultivars a spectral database was created as a tool for confirmation of substances. So far, 66 fragmentation spectra of phytocannabinoids and 12 fragmentation spectra of other biologically active substances have been obtained.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů