Application of the LAMP method for food pathogens detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919566" target="_blank" >RIV/60461373:22330/19:43919566 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/19:43919577

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Application of the LAMP method for food pathogens detection

Popis výsledku v původním jazyce

The LAMP method (‘loop-mediated isothermal amplification’) is a modern method for rapid and conclusive detection of pathogenic or indicator microorganisms in various food matrices with considerable potential for the future. Its main advantages include speed, robustness, high specificity and selectivity of its reaction. The aim of this work is application of the LAMP method for fast and reliable detection of food pathogens in various food matrices purposefully contaminated under laboratory conditions. The LAMP method was used for the experiment with 10 food matrices based on following methodology. Three samples were prepared from each matrix: one sample was control and two samples were contaminated by Escherichia coli O157:H7 ATCC 43888 and Salmonella enterica subsp. enterica ATCC 51741 (inoculum 102 CFU g-1 food matrix). For the back-detection of pathogens were used so-called the LAMP ‘food panels‘ (the supplier: Amplex Biosystems GmbH, DEU). It was found that in 19 of 20 tested samples, intentionally contaminated by Salmonella enterica subsp. enterica ATCC 51741, was realized successful back detection from 95 % and that in 15 of 20 samples, intentionally contaminated by Escherichia coli O157: H7 ATCC 4388, was realized successful back detection from 75 %. The LAMP method can be used in the food industry at aimed protection of health safety of food raw materials and products.

Název v anglickém jazyce

Application of the LAMP method for food pathogens detection

Popis výsledku anglicky

The LAMP method (‘loop-mediated isothermal amplification’) is a modern method for rapid and conclusive detection of pathogenic or indicator microorganisms in various food matrices with considerable potential for the future. Its main advantages include speed, robustness, high specificity and selectivity of its reaction. The aim of this work is application of the LAMP method for fast and reliable detection of food pathogens in various food matrices purposefully contaminated under laboratory conditions. The LAMP method was used for the experiment with 10 food matrices based on following methodology. Three samples were prepared from each matrix: one sample was control and two samples were contaminated by Escherichia coli O157:H7 ATCC 43888 and Salmonella enterica subsp. enterica ATCC 51741 (inoculum 102 CFU g-1 food matrix). For the back-detection of pathogens were used so-called the LAMP ‘food panels‘ (the supplier: Amplex Biosystems GmbH, DEU). It was found that in 19 of 20 tested samples, intentionally contaminated by Salmonella enterica subsp. enterica ATCC 51741, was realized successful back detection from 95 % and that in 15 of 20 samples, intentionally contaminated by Escherichia coli O157: H7 ATCC 4388, was realized successful back detection from 75 %. The LAMP method can be used in the food industry at aimed protection of health safety of food raw materials and products.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/QK1710156" target="_blank" >QK1710156: Nové přístupy a metody analýzy pro zajištění kvality, bezpečnosti a zdravotní nezávadnosti sýrů, optimalizace jejich výroby a zefektivnění procesů hygieny a sanitace při současném snížení zátěže životního prostředí odpadními vodami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů