Authentication of opium poppy (Papaver somniferum L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919827" target="_blank" >RIV/60461373:22330/19:43919827 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Authentication of opium poppy (Papaver somniferum L.)

Popis výsledku v původním jazyce

Opium poppy (Papaver somniferum L.) is an important oilseed using in the food and pharmaceutical industry. In the Czech Republic, the cultivation of P. somniferum L. has a long tradition. Czech poppy seeds have good nutritional and sensory quality and low levels of opiates. Therefore, poppy seeds are important ingredients in food items not only in the Czech Republic but also in many countries of Central-Eastern Europe. They are widely used for the preparation of pastry. Recently, there have been several cases of poppy seed falsification. Food poppy seeds were mixed with cheaper seeds from pharmaceutical varieties that contain a higher amount of opium alkaloids and have worse sensory properties. This can lead not only to deceiving the customer but also to health problems. Therefore, it is important to find a way to detect food adulteration. One possibility is to use microscopy, e.g. SEM, but it may not be sufficient for a mixture of crushed poppies. Thus, we tested the possibility to differentiate P. somniferum not only by microscopy but also by DNA analysis of genes important for the biosynthetic pathway of opium alkaloids. Microscopy allows distinguishing some ornamental poppies from P. somniferum. DNA analysis was performed using a polymerase chain reaction. The selected sequence of the COR gene allows distinguishing P. somniferum from other tested poppy species. REPI gene seems to be suitable for the differentiation of poppy varieties with low- and high-alkaloid content.

Název v anglickém jazyce

Authentication of opium poppy (Papaver somniferum L.)

Popis výsledku anglicky

Opium poppy (Papaver somniferum L.) is an important oilseed using in the food and pharmaceutical industry. In the Czech Republic, the cultivation of P. somniferum L. has a long tradition. Czech poppy seeds have good nutritional and sensory quality and low levels of opiates. Therefore, poppy seeds are important ingredients in food items not only in the Czech Republic but also in many countries of Central-Eastern Europe. They are widely used for the preparation of pastry. Recently, there have been several cases of poppy seed falsification. Food poppy seeds were mixed with cheaper seeds from pharmaceutical varieties that contain a higher amount of opium alkaloids and have worse sensory properties. This can lead not only to deceiving the customer but also to health problems. Therefore, it is important to find a way to detect food adulteration. One possibility is to use microscopy, e.g. SEM, but it may not be sufficient for a mixture of crushed poppies. Thus, we tested the possibility to differentiate P. somniferum not only by microscopy but also by DNA analysis of genes important for the biosynthetic pathway of opium alkaloids. Microscopy allows distinguishing some ornamental poppies from P. somniferum. DNA analysis was performed using a polymerase chain reaction. The selected sequence of the COR gene allows distinguishing P. somniferum from other tested poppy species. REPI gene seems to be suitable for the differentiation of poppy varieties with low- and high-alkaloid content.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40101 - Agriculture

Projekt

<a href="/cs/project/QK1720263" target="_blank" >QK1720263: Diagnostické metody pro laboratorní kontrolu pravosti máku setého</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů