PURIFICATION OF WATER-SOLUBLE BIOACTIVE POLYSACCHARIDES FROM BIOMASS OF HETEROTROPHIC MUTANT ALGA CHLORELLA VULGARIS G11

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43920813" target="_blank" >RIV/60461373:22330/19:43920813 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/19:00534790

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

PURIFICATION OF WATER-SOLUBLE BIOACTIVE POLYSACCHARIDES FROM BIOMASS OF HETEROTROPHIC MUTANT ALGA CHLORELLA VULGARIS G11

Popis výsledku v původním jazyce

Water extract obtained from biomass of mutant alga Chlorella vulgaris G11 was deproteinized and then purified with anion-exchange chromatography (AEC) on DEAE Sepharose Fast Flow column and gel filtration chromatography on Sepaphadex G-75 superfine column. Two high MW (&gt;1000 kDa and 98.3 kDa) polysaccharides (CPS1 and CPS3) were eluted with water and 0.5M NaCl on DEAE column and heteropolysaccharide with MW of 15 kDa (CPS2) was also eluted with water on DEAE. The former fractions were identified as branched rhamnan; the latter one as branched xylogalactofuranan of unusual structure. CPS1 showed properties of an unstable polymer: according to GPC analysis and by using light scattering and RI detectors it was found that one fraction of polymers (260-800 kDa) has self-assembling properties in aqueous solution and MW of this fraction decreased during some time. On the other hand, polymer with initially larger MW (&gt;1000 kDa) became even bigger. CPS3 was eluted with 0.3-0.5 M of NaCl on DEAE Sepharose FF column and have MW of 98.3 kDa. Cell toxicity of the products was tested in vitro on cell cultures for different concentrations.

Název v anglickém jazyce

PURIFICATION OF WATER-SOLUBLE BIOACTIVE POLYSACCHARIDES FROM BIOMASS OF HETEROTROPHIC MUTANT ALGA CHLORELLA VULGARIS G11

Popis výsledku anglicky

Water extract obtained from biomass of mutant alga Chlorella vulgaris G11 was deproteinized and then purified with anion-exchange chromatography (AEC) on DEAE Sepharose Fast Flow column and gel filtration chromatography on Sepaphadex G-75 superfine column. Two high MW (&gt;1000 kDa and 98.3 kDa) polysaccharides (CPS1 and CPS3) were eluted with water and 0.5M NaCl on DEAE column and heteropolysaccharide with MW of 15 kDa (CPS2) was also eluted with water on DEAE. The former fractions were identified as branched rhamnan; the latter one as branched xylogalactofuranan of unusual structure. CPS1 showed properties of an unstable polymer: according to GPC analysis and by using light scattering and RI detectors it was found that one fraction of polymers (260-800 kDa) has self-assembling properties in aqueous solution and MW of this fraction decreased during some time. On the other hand, polymer with initially larger MW (&gt;1000 kDa) became even bigger. CPS3 was eluted with 0.3-0.5 M of NaCl on DEAE Sepharose FF column and have MW of 98.3 kDa. Cell toxicity of the products was tested in vitro on cell cultures for different concentrations.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/FV10155" target="_blank" >FV10155: Řasové biotechnologie pro potravinářství - příprava proteinů, polysacharidů a karotenů heterotrofní kultivací mikrořas se sníženým množstvím chlorofylu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

PROCEEDINGS OF THE 15TH INTERNATIONAL CONFERENCE ON POLYSACCHARIDES-GLYCOSCIENCE

ISBN

978-80-88307-04-4

ISSN

2336-6796

e-ISSN

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Počet stran výsledku

6

Strana od-do

158-163

Název nakladatele

Česká společnost chemická

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

13. 11. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000544671400037