Izolace ?pathogenesis-related? proteinů z tabáku infikovaného virem TMV a jejich vliv na infektivitu viru TMV
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F05%3A00028420" target="_blank" >RIV/61389030:_____/05:00028420 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Isolation of pathogenesis-related proteins from TMV-Infected tobacco and their influence on infectivity of TMV
Popis výsledku v původním jazyce
The composition of pathogenesis-related proteins (PR-proteins) and inhibitory influence on TMV multiplication were studied. The ICF PR-proteins of infected plants were separated after solubilisation by decreasing gradient of ammonium sulphate, the cell PR-proteins were separated after acidic homogenisation of leaf tissues. The ICF and cell PR-proteins were further purified by ion exchange chromatography on DEAE cellulose. Using discontinuous non-denaturating polyacrylamide gel electrophoresis of DEAE cellulose fractions the PR-proteins were detected. Molecular weights were estimated by SDS-PAGE. The ICF and cell proteins of infected leaves included PR-proteins of the molecular weights 15?16 kDa (Group 1), 27?28 kDa (Group 3: chitinases) and 36?40 kDa (Group 2a: ?-1,3-glucanases). Fractions with different PR-proteins were tested for their effect on infectivity of TMV. Particularly the PR3 and PR2a proteins seem to decrease the infectivity of TMV.
Název v anglickém jazyce
Isolation of pathogenesis-related proteins from TMV-Infected tobacco and their influence on infectivity of TMV
Popis výsledku anglicky
The composition of pathogenesis-related proteins (PR-proteins) and inhibitory influence on TMV multiplication were studied. The ICF PR-proteins of infected plants were separated after solubilisation by decreasing gradient of ammonium sulphate, the cell PR-proteins were separated after acidic homogenisation of leaf tissues. The ICF and cell PR-proteins were further purified by ion exchange chromatography on DEAE cellulose. Using discontinuous non-denaturating polyacrylamide gel electrophoresis of DEAE cellulose fractions the PR-proteins were detected. Molecular weights were estimated by SDS-PAGE. The ICF and cell proteins of infected leaves included PR-proteins of the molecular weights 15?16 kDa (Group 1), 27?28 kDa (Group 3: chitinases) and 36?40 kDa (Group 2a: ?-1,3-glucanases). Fractions with different PR-proteins were tested for their effect on infectivity of TMV. Particularly the PR3 and PR2a proteins seem to decrease the infectivity of TMV.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA522%2F02%2F0708" target="_blank" >GA522/02/0708: Studium specifické role chloroplastů v mechanismech replikace a translace virové RND ve vztahu k resistenci hostitele</a><br>
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2005
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Plant Protection Science
ISSN
1212-2580
e-ISSN
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Svazek periodika
41
Číslo periodika v rámci svazku
-
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
6
Strana od-do
52-57
Kód UT WoS článku
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EID výsledku v databázi Scopus
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