Isolation and identification of Pantoea agglomerans from the inflated bag with dried hop pellets stored under a modified atmosphere

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43921031" target="_blank" >RIV/60461373:22330/21:43921031 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/20:43921031 RIV/14864347:_____/21:N0000001

Výsledek na webu

<a href="https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/jam.14970" target="_blank" >https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/jam.14970</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jam.14970" target="_blank" >10.1111/jam.14970</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Isolation and identification of Pantoea agglomerans from the inflated bag with dried hop pellets stored under a modified atmosphere

Popis výsledku v původním jazyce

Aims Isolation, characterization and identification of possible microbial contaminant(s) in the inflated foil bag containing hop pellets packed and stored in a modified atmosphere. Methods and results Package gas of the inflated foil bag containing hop pellets was analysed by gas chromatography. Compared with the reference modified atmosphere, containing about 16 vol.% of CO2, the inflated bag atmosphere contained 53 vol.% CO2, suggesting possible microbial contamination. Therefore, several standard and mineral media, with added hop pellets or hop infusion, were used for cultivation at different temperatures under an anaerobic atmosphere. Cultivation in mineral medium with hop pellets yielded a bacterial isolate that was identified by MALDI-TOF mass spectrometry and verified by partial 16S rRNA gene analysis as Pantoea agglomerans, a known plant epiphyte. Conclusions A novel strain of P. agglomerans (designed as DBM 3696) was found to be suspicious of causing inflation of the foil bag containing dried hop pellets packed in modified atmosphere. Significance and Impact of the Study This study suggests that P. agglomerans, probably hop epiphyte, could cause sporadic inflation of bags with hop pellets packed in modified atmosphere causing logistical problems during bags transport.

Název v anglickém jazyce

Isolation and identification of Pantoea agglomerans from the inflated bag with dried hop pellets stored under a modified atmosphere

Popis výsledku anglicky

Aims Isolation, characterization and identification of possible microbial contaminant(s) in the inflated foil bag containing hop pellets packed and stored in a modified atmosphere. Methods and results Package gas of the inflated foil bag containing hop pellets was analysed by gas chromatography. Compared with the reference modified atmosphere, containing about 16 vol.% of CO2, the inflated bag atmosphere contained 53 vol.% CO2, suggesting possible microbial contamination. Therefore, several standard and mineral media, with added hop pellets or hop infusion, were used for cultivation at different temperatures under an anaerobic atmosphere. Cultivation in mineral medium with hop pellets yielded a bacterial isolate that was identified by MALDI-TOF mass spectrometry and verified by partial 16S rRNA gene analysis as Pantoea agglomerans, a known plant epiphyte. Conclusions A novel strain of P. agglomerans (designed as DBM 3696) was found to be suspicious of causing inflation of the foil bag containing dried hop pellets packed in modified atmosphere. Significance and Impact of the Study This study suggests that P. agglomerans, probably hop epiphyte, could cause sporadic inflation of bags with hop pellets packed in modified atmosphere causing logistical problems during bags transport.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/TE02000177" target="_blank" >TE02000177: Centrum pro inovativní využití a posílení konkurenceschopnosti českých pivovarských surovin a výrobků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Microbiology

ISSN

1364-5072

e-ISSN

—

Svazek periodika

Neuveden

Číslo periodika v rámci svazku

JUL 2021

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

281-287

Kód UT WoS článku

000602613700001

EID výsledku v databázi Scopus

2-s2.0-85098226695