Regulated and non-regulated mycotoxin detection in cereal matrices using an ultra-high-performance liquid chromatography high-resolution mass spectrometry (Uhplc-hrms) method
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43922796" target="_blank" >RIV/60461373:22330/21:43922796 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.mdpi.com/2072-6651/13/11/783" target="_blank" >https://www.mdpi.com/2072-6651/13/11/783</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/toxins13110783" target="_blank" >10.3390/toxins13110783</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Regulated and non-regulated mycotoxin detection in cereal matrices using an ultra-high-performance liquid chromatography high-resolution mass spectrometry (Uhplc-hrms) method
Popis výsledku v původním jazyce
Cereals represent a widely consumed food commodity that might be contaminated by mycotoxins, resulting not only in potential consumer health risks upon dietary exposure but also significant financial losses due to contaminated batch disposal. Thus, continuous improvement of the performance characteristics of methods to enable an effective monitoring of such contaminants in food supply is highly needed. In this study, an ultra-high-performance liquid chromatography coupled to a hybrid quadrupole orbitrap mass analyzer (UHPLC-q-Orbitrap MS) method was optimized and validated in wheat, maize and rye flour matrices. Nineteen analytes were monitored, including both regulated mycotoxins, e.g., ochratoxin A (OTA) or deoxynivalenol (DON), and non-regulated mycotoxins, such as ergot alkaloids (EAs), which are analytes that are expected to be regulated soon in the EU. Low limits of quantification (LOQ) at the part per trillion level were achieved as well as wide linear ranges (four orders of magnitude) and recovery rates within the 68–104% range. Overall, the developed method attained fit-for-purpose results and it highlights the applicability of high-resolution mass spectrometry (HRMS) detection in mycotoxin food analysis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Název v anglickém jazyce
Regulated and non-regulated mycotoxin detection in cereal matrices using an ultra-high-performance liquid chromatography high-resolution mass spectrometry (Uhplc-hrms) method
Popis výsledku anglicky
Cereals represent a widely consumed food commodity that might be contaminated by mycotoxins, resulting not only in potential consumer health risks upon dietary exposure but also significant financial losses due to contaminated batch disposal. Thus, continuous improvement of the performance characteristics of methods to enable an effective monitoring of such contaminants in food supply is highly needed. In this study, an ultra-high-performance liquid chromatography coupled to a hybrid quadrupole orbitrap mass analyzer (UHPLC-q-Orbitrap MS) method was optimized and validated in wheat, maize and rye flour matrices. Nineteen analytes were monitored, including both regulated mycotoxins, e.g., ochratoxin A (OTA) or deoxynivalenol (DON), and non-regulated mycotoxins, such as ergot alkaloids (EAs), which are analytes that are expected to be regulated soon in the EU. Low limits of quantification (LOQ) at the part per trillion level were achieved as well as wide linear ranges (four orders of magnitude) and recovery rates within the 68–104% range. Overall, the developed method attained fit-for-purpose results and it highlights the applicability of high-resolution mass spectrometry (HRMS) detection in mycotoxin food analysis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Klasifikace
Druh
J<sub>SC</sub> - Článek v periodiku v databázi SCOPUS
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/LM2018100" target="_blank" >LM2018100: Infrastruktura pro propagaci metrologie v potravinářství a výživě v České republice</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Toxins
ISSN
2072-6651
e-ISSN
—
Svazek periodika
13
Číslo periodika v rámci svazku
11
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
14
Strana od-do
nestrankovano
Kód UT WoS článku
—
EID výsledku v databázi Scopus
2-s2.0-85119272938