Identification of homologous polyprenols from thermophilic bacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43922991" target="_blank" >RIV/60461373:22330/21:43922991 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/21:00546635 RIV/60193697:_____/21:N0000019

Výsledek na webu

<a href="https://www.mdpi.com/2076-2607/9/6/1168/htm" target="_blank" >https://www.mdpi.com/2076-2607/9/6/1168/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms9061168" target="_blank" >10.3390/microorganisms9061168</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of homologous polyprenols from thermophilic bacteria

Popis výsledku v původním jazyce

Sixteen strains of five genera of thermophilic bacteria, i.e., Alicyclobacillus, Brevibacillus, Geobacillus, Meiothermus, and Thermus, were cultivated at a temperature from 42 to 70 °C. Twelve strains were obtained from the Czech Collection of Microorganisms, while four were directly isolated and identified by 16S rRNA gene sequencing from the hot springs of the world‐famous Carlsbad spa (Czech Republic). Polyprenol homologs from C40 to C65 as well as free undecaprenol (C55), undecaprenyl phosphate, and undecaprenyl diphosphate were identified by shotgun analysis and RP‐HPLC/MS‐ESI+ (reverse phase high‐performance liquid chromatography–high‐resolution positive electrospray ionization mass spectrometry). The limit of detection (50 pM) was determined for individual homologs and free polyprenols and their phosphates. Thus, it has been shown that at least some thermophilic bacteria produce not just the major C55 polyprenol as previously described, but a mixture of homologs. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Název v anglickém jazyce

Identification of homologous polyprenols from thermophilic bacteria

Popis výsledku anglicky

Sixteen strains of five genera of thermophilic bacteria, i.e., Alicyclobacillus, Brevibacillus, Geobacillus, Meiothermus, and Thermus, were cultivated at a temperature from 42 to 70 °C. Twelve strains were obtained from the Czech Collection of Microorganisms, while four were directly isolated and identified by 16S rRNA gene sequencing from the hot springs of the world‐famous Carlsbad spa (Czech Republic). Polyprenol homologs from C40 to C65 as well as free undecaprenol (C55), undecaprenyl phosphate, and undecaprenyl diphosphate were identified by shotgun analysis and RP‐HPLC/MS‐ESI+ (reverse phase high‐performance liquid chromatography–high‐resolution positive electrospray ionization mass spectrometry). The limit of detection (50 pM) was determined for individual homologs and free polyprenols and their phosphates. Thus, it has been shown that at least some thermophilic bacteria produce not just the major C55 polyprenol as previously described, but a mixture of homologs. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20801 - Environmental biotechnology

Projekt

<a href="/cs/project/GA18-00036S" target="_blank" >GA18-00036S: Ekologie extrémofilních mikroorganismů ve vodách kulturně významných českých pramenů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microorganisms

ISSN

2076-2607

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

—

Kód UT WoS článku

000666057600001

EID výsledku v databázi Scopus

2-s2.0-85106602333