Tailor-made immunochromatographic test for the detection of multiple 17 alpha-methylated anabolics in dietary supplements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43923282" target="_blank" >RIV/60461373:22330/21:43923282 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2304-8158/10/4/741/htm" target="_blank" >https://www.mdpi.com/2304-8158/10/4/741/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/foods10040741" target="_blank" >10.3390/foods10040741</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Tailor-made immunochromatographic test for the detection of multiple 17 alpha-methylated anabolics in dietary supplements

Popis výsledku v původním jazyce

The presented study describes the development of an immunostrip test for the detection of multiple 17 alpha-methylated AAS based on direct and indirect competitive principle using gold nanoparticles as a label. As a capture reagent on test lines conjugated stanazolol to rabbit serum albumin (RSA/ST-3) was used, the intensity of color formed in the test line of the AAS-positive sample was visually distinguishable from that of negative sample within 10 min. The optimized closed direct and indirect format of the test provided a similar visual detection limit (0.7 and 0.9 ng/mL, respectively). The most commonly orally abused AAS (17 alpha-methyltestosterone, methandienone, methyldihydrotestosterone, oxandrolone and oxymetholone) showed a strong cross-reaction. Developed immunostrips were successfully applied to analysis of artificially contaminated dietary supplements with 17 alpha-methylated AASs. The developed immunostrips offer potential as a useful user-friendly method for capturing suspicious dietary supplement samples with different contents of AAS at levels far below the usually used concentrations of AAS.

Název v anglickém jazyce

Tailor-made immunochromatographic test for the detection of multiple 17 alpha-methylated anabolics in dietary supplements

Popis výsledku anglicky

The presented study describes the development of an immunostrip test for the detection of multiple 17 alpha-methylated AAS based on direct and indirect competitive principle using gold nanoparticles as a label. As a capture reagent on test lines conjugated stanazolol to rabbit serum albumin (RSA/ST-3) was used, the intensity of color formed in the test line of the AAS-positive sample was visually distinguishable from that of negative sample within 10 min. The optimized closed direct and indirect format of the test provided a similar visual detection limit (0.7 and 0.9 ng/mL, respectively). The most commonly orally abused AAS (17 alpha-methyltestosterone, methandienone, methyldihydrotestosterone, oxandrolone and oxymetholone) showed a strong cross-reaction. Developed immunostrips were successfully applied to analysis of artificially contaminated dietary supplements with 17 alpha-methylated AASs. The developed immunostrips offer potential as a useful user-friendly method for capturing suspicious dietary supplement samples with different contents of AAS at levels far below the usually used concentrations of AAS.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Foods

ISSN

2304-8158

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

1-15

Kód UT WoS článku

000643048600001

EID výsledku v databázi Scopus

2-s2.0-85104296720