Detection of Botanical Adulterants in Powdered Saffron

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925037" target="_blank" >RIV/60461373:22330/22:43925037 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of Botanical Adulterants in Powdered Saffron

Popis výsledku v původním jazyce

Saffron is a spice obtained by drying the stigmas of the saffron flowers (Crocus sativus L.). Due to its high price, economically motivated adulteration occurs relatively often. For example, powdered saffron is adulterated by mixing with other plant materials or extracts thereof. Based on the spectrophotometric methods given in the international standards ISO 3632-1:2011 and ISO 3632- 2:2010 dealing with the quality and classification of saffron, it is possible to detect 10-20% weight additions of safflower, marigold, and turmeric to saffron. The presented study aimed to develop an effective strategy for the detection of more potential botanical adulterants in saffron, namely safflower (Carthamus tinctorius L.), marigold (Calendula officinalis L.), turmeric (Curcuma longa L.), achiote (Bixa orellana L.), red pepper (Capsicum spp.), mountain arnica (Arnica montana L.), beet (Beta vulgaris L.) and pomegranate (Punica granatum L.). Non-target screening strategy based on ultra-high reverse-phase liquid chromatography coupled to tandem high-resolution mass spectrometry (UHPLC-HRMS/MS) was employed for the analysis of plant extracts (obtained by aqueous ethanol). By using multivariate statistical analysis of the generated ´chemical fingerprints´, specifically using the method of principal components (PCA) and partial least squares-discriminant analysis (PLS-DA), potential unique markers (metabolites) could be identified. To enable routine saffron authenticity control by target screening, the internal database was developed, currently it involves 82 unique markers (both new ones were possible reliably discovered in this study and those reported in the literature). In this way, detection of 1 – 3% (w/w) addition of all analyzed botanical adulterants was possible.

Název v anglickém jazyce

Detection of Botanical Adulterants in Powdered Saffron

Popis výsledku anglicky

Saffron is a spice obtained by drying the stigmas of the saffron flowers (Crocus sativus L.). Due to its high price, economically motivated adulteration occurs relatively often. For example, powdered saffron is adulterated by mixing with other plant materials or extracts thereof. Based on the spectrophotometric methods given in the international standards ISO 3632-1:2011 and ISO 3632- 2:2010 dealing with the quality and classification of saffron, it is possible to detect 10-20% weight additions of safflower, marigold, and turmeric to saffron. The presented study aimed to develop an effective strategy for the detection of more potential botanical adulterants in saffron, namely safflower (Carthamus tinctorius L.), marigold (Calendula officinalis L.), turmeric (Curcuma longa L.), achiote (Bixa orellana L.), red pepper (Capsicum spp.), mountain arnica (Arnica montana L.), beet (Beta vulgaris L.) and pomegranate (Punica granatum L.). Non-target screening strategy based on ultra-high reverse-phase liquid chromatography coupled to tandem high-resolution mass spectrometry (UHPLC-HRMS/MS) was employed for the analysis of plant extracts (obtained by aqueous ethanol). By using multivariate statistical analysis of the generated ´chemical fingerprints´, specifically using the method of principal components (PCA) and partial least squares-discriminant analysis (PLS-DA), potential unique markers (metabolites) could be identified. To enable routine saffron authenticity control by target screening, the internal database was developed, currently it involves 82 unique markers (both new ones were possible reliably discovered in this study and those reported in the literature). In this way, detection of 1 – 3% (w/w) addition of all analyzed botanical adulterants was possible.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LM2018100" target="_blank" >LM2018100: Infrastruktura pro propagaci metrologie v potravinářství a výživě v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů