Investigating the potential of berry plant extracts to inhibit pancreatic lipase: Combining in vitro assays to metabolomic suspect screening

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925315" target="_blank" >RIV/60461373:22330/22:43925315 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Investigating the potential of berry plant extracts to inhibit pancreatic lipase: Combining in vitro assays to metabolomic suspect screening

Popis výsledku v původním jazyce

Pancreatic lipase (PNLIP, EC 3.1.1.3) plays a pivotal role in the digestion of dietary lipids, a metabolic pathway strictly related to obesity. An effective strategy in obesity treatment is the inhibition of PNLIP, which is possible to be achieved by phytochemicals found in high abundance in plants [1]. In this study, a multidisciplinary approach is presented investigating the in vitro PNLIP inhibitory effect of 8 berry plant extracts belonging to the Ericaceae, Grossulariaceae and Rosaceae families, plants with proven anti-obesity potential [2]. Initially, a rapid and cost-efficient assay PNLIP 96-microwell plate assay was developed and important parameters were optimized, e.g., the enzyme substrate. The optimized assay was used to monitor the inhibitory effect of both aqueous and dichloromethane (DCM) extracts. Importantly, half of the tested aqueous extracts induced an inhibition rate higher than 60% (n=4) with blackcurrant (Ribes nigrum) and bearberry (Arctostaphylos uva ursi) extracts (10 mg mL-1) almost completely inhibiting PNLIP activity. In the case of the DCM fractions, the monitored in vitro inhibitory effect was much lower with blackberry leaves achieving the highest inhibition rate (44% ± 5%, n=4). Considering the significantly higher inhibitory effect of the aqueous fractions, it can be assumed that polar compounds were more potent to inhibit the PNLIP activity. In the next stage of the experiment, to tentatively identify the composition of the tested berry fruits a metabolomic suspect screening workflow was followed using a quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) analyzer. Briefly, an in-house database containing 217 natural bioactive compounds, e.g., polyphenols and alkaloids, was screened and work is under-way to complete the chemical characterisation of the analysed extracts. All in all, the presented approach combines in vitro bioactivity measurements to high-end metabolomics to identify molecules with potential medicinal and/or dietary applications.

Název v anglickém jazyce

Investigating the potential of berry plant extracts to inhibit pancreatic lipase: Combining in vitro assays to metabolomic suspect screening

Popis výsledku anglicky

Pancreatic lipase (PNLIP, EC 3.1.1.3) plays a pivotal role in the digestion of dietary lipids, a metabolic pathway strictly related to obesity. An effective strategy in obesity treatment is the inhibition of PNLIP, which is possible to be achieved by phytochemicals found in high abundance in plants [1]. In this study, a multidisciplinary approach is presented investigating the in vitro PNLIP inhibitory effect of 8 berry plant extracts belonging to the Ericaceae, Grossulariaceae and Rosaceae families, plants with proven anti-obesity potential [2]. Initially, a rapid and cost-efficient assay PNLIP 96-microwell plate assay was developed and important parameters were optimized, e.g., the enzyme substrate. The optimized assay was used to monitor the inhibitory effect of both aqueous and dichloromethane (DCM) extracts. Importantly, half of the tested aqueous extracts induced an inhibition rate higher than 60% (n=4) with blackcurrant (Ribes nigrum) and bearberry (Arctostaphylos uva ursi) extracts (10 mg mL-1) almost completely inhibiting PNLIP activity. In the case of the DCM fractions, the monitored in vitro inhibitory effect was much lower with blackberry leaves achieving the highest inhibition rate (44% ± 5%, n=4). Considering the significantly higher inhibitory effect of the aqueous fractions, it can be assumed that polar compounds were more potent to inhibit the PNLIP activity. In the next stage of the experiment, to tentatively identify the composition of the tested berry fruits a metabolomic suspect screening workflow was followed using a quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) analyzer. Briefly, an in-house database containing 217 natural bioactive compounds, e.g., polyphenols and alkaloids, was screened and work is under-way to complete the chemical characterisation of the analysed extracts. All in all, the presented approach combines in vitro bioactivity measurements to high-end metabolomics to identify molecules with potential medicinal and/or dietary applications.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30202 - Endocrinology and metabolism (including diabetes, hormones)

Projekt

<a href="/cs/project/LM2018100" target="_blank" >LM2018100: Infrastruktura pro propagaci metrologie v potravinářství a výživě v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů