Identifying edible plants with high anti-obesity potential: In vitro inhibitory effect against pancreatic lipase followed by bioactive compound metabolomic screening

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F23%3A43928182" target="_blank" >RIV/60461373:22330/23:43928182 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/23:43926317 RIV/00064173:_____/23:43926317

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2212429223011045?via%3Dihub#ack0010" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2212429223011045?via%3Dihub#ack0010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fbio.2023.103453" target="_blank" >10.1016/j.fbio.2023.103453</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identifying edible plants with high anti-obesity potential: In vitro inhibitory effect against pancreatic lipase followed by bioactive compound metabolomic screening

Popis výsledku v původním jazyce

The ever-growing number of individuals facing obesity related health problems represents an alarming situation. Within the available obesity control strategies, there is evidence that some natural compounds can inhibit pancreatic lipase (PNLIP, EC 3.1.1.3), a digestive enzyme with pivotal role in the hydrolysis of dietary triacylglycerols, resulting in reduced fat metabolism. In this study, to identify sources of bioactive compounds with anti-obesity potential, 278 extracts originating by 126 food species were prepared in water and dichloromethane (DCM) and their in vitro inhibitory potential against PNLIP was measured. Importantly, 12 extracts exceeded the set cut-off level of significant inhibitory effect (inhibition&gt; 70%) and for them dose-response curves were prepared and metabolomic target screening was performed. By using ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS), it was possible to tentatively identify 28 and 22 secondary metabolites in the aqueous and DCM extracts, respectively. Several species-specific compounds were detected, e.g., hesperidin and eriocitrin in lemon and lime fruit aqueous extracts or curcuminoids in DCM turmeric extracts. Additionally, the highest percentage of the total analytical signal detected in dill, walnut, caper and bell pepper DCM extracts corresponded to various fatty acids. All in all, the edible plant extracts with strong anti-obesity potential contained various bioactive phytochemicals that may be used in nutraceutical applications. © 2023 Elsevier Ltd

Název v anglickém jazyce

Identifying edible plants with high anti-obesity potential: In vitro inhibitory effect against pancreatic lipase followed by bioactive compound metabolomic screening

Popis výsledku anglicky

The ever-growing number of individuals facing obesity related health problems represents an alarming situation. Within the available obesity control strategies, there is evidence that some natural compounds can inhibit pancreatic lipase (PNLIP, EC 3.1.1.3), a digestive enzyme with pivotal role in the hydrolysis of dietary triacylglycerols, resulting in reduced fat metabolism. In this study, to identify sources of bioactive compounds with anti-obesity potential, 278 extracts originating by 126 food species were prepared in water and dichloromethane (DCM) and their in vitro inhibitory potential against PNLIP was measured. Importantly, 12 extracts exceeded the set cut-off level of significant inhibitory effect (inhibition&gt; 70%) and for them dose-response curves were prepared and metabolomic target screening was performed. By using ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS), it was possible to tentatively identify 28 and 22 secondary metabolites in the aqueous and DCM extracts, respectively. Several species-specific compounds were detected, e.g., hesperidin and eriocitrin in lemon and lime fruit aqueous extracts or curcuminoids in DCM turmeric extracts. Additionally, the highest percentage of the total analytical signal detected in dill, walnut, caper and bell pepper DCM extracts corresponded to various fatty acids. All in all, the edible plant extracts with strong anti-obesity potential contained various bioactive phytochemicals that may be used in nutraceutical applications. © 2023 Elsevier Ltd

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30308 - Nutrition, Dietetics

Projekt

<a href="/cs/project/NU21-01-00259" target="_blank" >NU21-01-00259: Nové inhibitory lipolýzy v léčbě poruch metabolismu lipidů a glukózy při syndromu obstrukční spánkové apnoe</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Bioscience

ISSN

2212-4292

e-ISSN

—

Svazek periodika

56

Číslo periodika v rámci svazku

103453

Stát vydavatele periodika

ZA - Jihoafrická republika

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

001141618200001

EID výsledku v databázi Scopus

2-s2.0-85180112860