DETERMINATION OF METHODOLOGY FOR MONITORING BIOFILM FORMATION OF FILAMENTOUS MICROSCOPIC FUNGI

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925735" target="_blank" >RIV/60461373:22330/22:43925735 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.icct.cz/cs/Amca-ICCT/media/content/2022/proceedings/ICCT2022-Proceedings.pdf" target="_blank" >https://www.icct.cz/cs/Amca-ICCT/media/content/2022/proceedings/ICCT2022-Proceedings.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

DETERMINATION OF METHODOLOGY FOR MONITORING BIOFILM FORMATION OF FILAMENTOUS MICROSCOPIC FUNGI

Popis výsledku v původním jazyce

Biofilm is a widespread survival strategy for microorganisms. In this form, they are protected from a wide range of adverse conditions (UV radiation, antimicrobials, drying out). For humans, biofilms may be desirable in industrial processes (biofiltration, bioremediation, wastewater treatment plants) or, conversely, cause significant problems (food contamination, deterioration of materials, reduced heat conduction efficiency). Unlike bacterial and yeast biofilms, filamentous microscopic fungal biofilms are neglected. This work focuses on the determination of a methodology suitable for the quantification of filamentous fungal biofilms. Three methods were selected, which are used for quantification of bacterial biofilms, and subsequently tested on single species biofilms of filamentous fungi Alternaria alternata DBM 4004, Aspergillus niger DBM 4054,Fusarium culmorum DBM 4044 and Fusarium graminearum DBM 4344. Crystal violet staining for the determination of total biofilm biomass and the MTT assay for the determination of metabolic activity of fungal biofilm were found to be appropriate for the quantification of filamentous fungal biofilm

Název v anglickém jazyce

DETERMINATION OF METHODOLOGY FOR MONITORING BIOFILM FORMATION OF FILAMENTOUS MICROSCOPIC FUNGI

Popis výsledku anglicky

Biofilm is a widespread survival strategy for microorganisms. In this form, they are protected from a wide range of adverse conditions (UV radiation, antimicrobials, drying out). For humans, biofilms may be desirable in industrial processes (biofiltration, bioremediation, wastewater treatment plants) or, conversely, cause significant problems (food contamination, deterioration of materials, reduced heat conduction efficiency). Unlike bacterial and yeast biofilms, filamentous microscopic fungal biofilms are neglected. This work focuses on the determination of a methodology suitable for the quantification of filamentous fungal biofilms. Three methods were selected, which are used for quantification of bacterial biofilms, and subsequently tested on single species biofilms of filamentous fungi Alternaria alternata DBM 4004, Aspergillus niger DBM 4054,Fusarium culmorum DBM 4044 and Fusarium graminearum DBM 4344. Crystal violet staining for the determination of total biofilm biomass and the MTT assay for the determination of metabolic activity of fungal biofilm were found to be appropriate for the quantification of filamentous fungal biofilm

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA22-13745S" target="_blank" >GA22-13745S: Zvýšení účinku biokontrolního agens Pythium oligandrum studenou plazmou proti plísňovým biofilmům</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 9th International Conference on Chemical Technology

ISBN

978-80-88307-11-2

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

382-385

Název nakladatele

Czech Society of Industrial Chemistry

Místo vydání

Prague

Místo konání akce

Mikulov

Datum konání akce

25. 4. 2022

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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