Analysis of mycotoxins and their glycosides by ultra-high performance liquid chromatography coupled with Q-orbitrap mass spectrometry

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of mycotoxins and their glycosides by ultra-high performance liquid chromatography coupled with Q-orbitrap mass spectrometry

Popis výsledku v původním jazyce

After infection of crops by microscopic filamentous fungi, mycotoxins are produced and further modified by plant enzymes. The most common mechanism of fusarium mycotoxins modification is conjugation with glucose, oligosaccharides and polysaccharides. Although this reduces the toxicity of mycotoxins to plants, enzymes present in the gastrointestinal tract or enzymes involved in certain food processing techniques are able to hydrolyse the glycosidic linkage between the carbohydrate and the mycotoxin. While the analysis of free mycotoxins is nowadays a common practice, the quantification of oligo-/polyglycosides is rather difficult, leading to underestimation of real health risk. There is thus an urgent need to develop effective approaches for their quantification.The aim of our study was to develop an analytical method for the indirect quantification of modified trichothecenes by enzymatic hydrolysis of the cereal matrix, and subsequently to characterize in detail the mono-/oligoglycosilated mycotoxins residues.To assure proper separation of isobaric isomers of various mycotoxins glycosides, ultra-high performance liquid chromatography with Acquity UPLC HSS T3 column (100 mm × 2,1 mm; 1,8 µm) was used. A high-resolution Q-orbitrap (Thermo Scientific) was utilized for mass spectrometric detection. For quantification, the data acquired in the fullMS mode (mass resolving power 70,000 FWHM) were primarily used. For peaks confirmation, high resolution MS/MS spectra of mycotoxins and their glycosides were acquired in paralel reaction monitoring mode (PRM), with reduced mass resolving power (17,500 FWHM).

Název v anglickém jazyce

Analysis of mycotoxins and their glycosides by ultra-high performance liquid chromatography coupled with Q-orbitrap mass spectrometry

Popis výsledku anglicky

After infection of crops by microscopic filamentous fungi, mycotoxins are produced and further modified by plant enzymes. The most common mechanism of fusarium mycotoxins modification is conjugation with glucose, oligosaccharides and polysaccharides. Although this reduces the toxicity of mycotoxins to plants, enzymes present in the gastrointestinal tract or enzymes involved in certain food processing techniques are able to hydrolyse the glycosidic linkage between the carbohydrate and the mycotoxin. While the analysis of free mycotoxins is nowadays a common practice, the quantification of oligo-/polyglycosides is rather difficult, leading to underestimation of real health risk. There is thus an urgent need to develop effective approaches for their quantification.The aim of our study was to develop an analytical method for the indirect quantification of modified trichothecenes by enzymatic hydrolysis of the cereal matrix, and subsequently to characterize in detail the mono-/oligoglycosilated mycotoxins residues.To assure proper separation of isobaric isomers of various mycotoxins glycosides, ultra-high performance liquid chromatography with Acquity UPLC HSS T3 column (100 mm × 2,1 mm; 1,8 µm) was used. A high-resolution Q-orbitrap (Thermo Scientific) was utilized for mass spectrometric detection. For quantification, the data acquired in the fullMS mode (mass resolving power 70,000 FWHM) were primarily used. For peaks confirmation, high resolution MS/MS spectra of mycotoxins and their glycosides were acquired in paralel reaction monitoring mode (PRM), with reduced mass resolving power (17,500 FWHM).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů