New possibilities in the analysis of modified trichothecenes type A in oats: immunoaffinity purification and enzymatic hydrolysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43921175" target="_blank" >RIV/60461373:22330/20:43921175 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

New possibilities in the analysis of modified trichothecenes type A in oats: immunoaffinity purification and enzymatic hydrolysis

Popis výsledku v původním jazyce

In the recent years, growing attention has been paid to modified mycotoxins, i.e. conjugates formed during plant detoxification processes or specific food technologies. In addition to glycosides of the most well-known trichothecene deoxynivalenol (DON), the presence of mono- / oligoglycosides of other trichothecenes, e.g. HT-2 / T-2 toxins (HT-2 / T-2), has also been published so far. The presence of these modified mycotoxins in a human diet has become an issue of health concern, which is underlined also by Scientific opinion issued by European Food Safety Authority in 2017. Whereas analytical standards for mycotoxin glycosides (except DON-3-glucoside) are not available, effective strategies for their isolation and detection are of major importance. In our study, we focused on quantification of free and modified HT-2 and T-2 in oat samples. Both direct analysis of free HT-2 / T-2, as well as indirect analysis of HT-2 after the enzymatic hydrolysis, were utilized (the glucosidase from Aspergillus niger used in our work was shown to effectively cleave the glycosidic bond in HT-2 glycosides only). Altogether, 52 oat samples were processed by using our previously developed purification and pre-concentration based on the immunoaffinity chromatography cross-reacting with the HT-2 / T-2 glycosides. The enzymatic hydrolysis was performed for the aliquot of the pre-concentrated extract. Analytical determination of both free mycotoxins, as well as their modified forms (both before and after hydrolysis), was realized by ultra-high performance liquid chromatography coupled to high resolution tandem mass spectrometry. Free T-2 was quantified in all 52 samples and its monoglucoside was present in 43 samples. In the case of HT-2, free form as well as its monoglucoside was present in 48 samples, and HT-2-diglucoside was detected in 29 samples. The percentage of HT-2 originally present in the form of glycosides from the total HT-2 content ranged from 0.1% to 56%.

Název v anglickém jazyce

New possibilities in the analysis of modified trichothecenes type A in oats: immunoaffinity purification and enzymatic hydrolysis

Popis výsledku anglicky

In the recent years, growing attention has been paid to modified mycotoxins, i.e. conjugates formed during plant detoxification processes or specific food technologies. In addition to glycosides of the most well-known trichothecene deoxynivalenol (DON), the presence of mono- / oligoglycosides of other trichothecenes, e.g. HT-2 / T-2 toxins (HT-2 / T-2), has also been published so far. The presence of these modified mycotoxins in a human diet has become an issue of health concern, which is underlined also by Scientific opinion issued by European Food Safety Authority in 2017. Whereas analytical standards for mycotoxin glycosides (except DON-3-glucoside) are not available, effective strategies for their isolation and detection are of major importance. In our study, we focused on quantification of free and modified HT-2 and T-2 in oat samples. Both direct analysis of free HT-2 / T-2, as well as indirect analysis of HT-2 after the enzymatic hydrolysis, were utilized (the glucosidase from Aspergillus niger used in our work was shown to effectively cleave the glycosidic bond in HT-2 glycosides only). Altogether, 52 oat samples were processed by using our previously developed purification and pre-concentration based on the immunoaffinity chromatography cross-reacting with the HT-2 / T-2 glycosides. The enzymatic hydrolysis was performed for the aliquot of the pre-concentrated extract. Analytical determination of both free mycotoxins, as well as their modified forms (both before and after hydrolysis), was realized by ultra-high performance liquid chromatography coupled to high resolution tandem mass spectrometry. Free T-2 was quantified in all 52 samples and its monoglucoside was present in 43 samples. In the case of HT-2, free form as well as its monoglucoside was present in 48 samples, and HT-2-diglucoside was detected in 29 samples. The percentage of HT-2 originally present in the form of glycosides from the total HT-2 content ranged from 0.1% to 56%.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů