Quantification of conjugated type A trichothecenes in cereals using immuno-affinity clean-up and enzymatic hydrolysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925452" target="_blank" >RIV/60461373:22330/22:43925452 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of conjugated type A trichothecenes in cereals using immuno-affinity clean-up and enzymatic hydrolysis

Popis výsledku v původním jazyce

Currently, increasing attention has been paid to conjugated mycotoxins in plant-based foods because of their assumed bioavailability and health risks. However, analytical standards for most mycotoxin glycosides are not available and risk assessment processes have not yet been completed because of the lack of occurrence data. Our study presents a method for efficient pre-concentration of type A trichothecene glycosides using immuno-affinity columns (IAC), followed by indirect determination after enzymatic hydrolysis. Cross-reactivity of antibodies of four types of commercially available IACs dedicated to HT-2/T-2 was tested for HT-2/T-2 glycosides. For enzymatic hydrolysis of β-glycosidic bond between HT-2/T-2 and releasing the saccharidic moiety, two different β- glycosidases were tested. Separation of free and glycosylated mycotoxins before and after hydrolysis was performed on Acquity UPLC® HSS T3 reversed phase column (100 mm x 2.1 mm, 1.8 mm; Waters), with subsequent detection assured by Q-orbitrap mass spectrometer in full-spectral acquisition mode with systematic fragmentation of precursor ions in ESI+ mode. This method was applied to 52 various oat-based products. Free T-2 toxin and T-2-monoglucosides were present in 92% and 69% of samples, respectively. Free HT-2 toxin and HT-2-monoglucosides were present in 92% of samples and HT-2-diglucosides were detected in 38% of samples. The percentage of HT-2 present in the form of glycosides was up to 56% of the total HT-2 content.

Název v anglickém jazyce

Quantification of conjugated type A trichothecenes in cereals using immuno-affinity clean-up and enzymatic hydrolysis

Popis výsledku anglicky

Currently, increasing attention has been paid to conjugated mycotoxins in plant-based foods because of their assumed bioavailability and health risks. However, analytical standards for most mycotoxin glycosides are not available and risk assessment processes have not yet been completed because of the lack of occurrence data. Our study presents a method for efficient pre-concentration of type A trichothecene glycosides using immuno-affinity columns (IAC), followed by indirect determination after enzymatic hydrolysis. Cross-reactivity of antibodies of four types of commercially available IACs dedicated to HT-2/T-2 was tested for HT-2/T-2 glycosides. For enzymatic hydrolysis of β-glycosidic bond between HT-2/T-2 and releasing the saccharidic moiety, two different β- glycosidases were tested. Separation of free and glycosylated mycotoxins before and after hydrolysis was performed on Acquity UPLC® HSS T3 reversed phase column (100 mm x 2.1 mm, 1.8 mm; Waters), with subsequent detection assured by Q-orbitrap mass spectrometer in full-spectral acquisition mode with systematic fragmentation of precursor ions in ESI+ mode. This method was applied to 52 various oat-based products. Free T-2 toxin and T-2-monoglucosides were present in 92% and 69% of samples, respectively. Free HT-2 toxin and HT-2-monoglucosides were present in 92% of samples and HT-2-diglucosides were detected in 38% of samples. The percentage of HT-2 present in the form of glycosides was up to 56% of the total HT-2 content.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LM2018100" target="_blank" >LM2018100: Infrastruktura pro propagaci metrologie v potravinářství a výživě v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů