Bioprospecting of Artemisia scoparia, Panax ginseng and Phellodendron sp.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F23%3A43928063" target="_blank" >RIV/60461373:22330/23:43928063 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Bioprospecting of Artemisia scoparia, Panax ginseng and Phellodendron sp.

Popis výsledku v původním jazyce

Many herbal extracts are known to contain biologically active molecules, some of which find uses in pharmaceuticals production. In the recent years, substances with a potential to inhibit lipolysis are being searched. One of potential applications is a treatment of obstructive sleep apnea, chronic disease which causes hypoxia and disrupts adipocyte metabolism. The aim of our study was to isolate and identify novel bioactive secondary metabolites from Artemisia scoparia, Panax ginseng and Phellodendron sp., which are presumed to contain lipolysis inhibitors. To obtain the entire set of compounds with different polarities, three types of extracts were prepared from each dried plant: aqueous, methanolic and dichloromethane extracts. These extracts were then fractionated using high performance liquid chromatography Waters AutoPurification System coupled to single quadrupole mass spectrometry detector (QDa). Nine sets of fractions were collected and their antilipolytic activity was measured. An enzymatic assay based on measurement of released free glycerol concentration was employed both for spontaneous and isoprenaline-induced lipolysis. Significant fractions with the highest inhibitory activity were further analyzed using ultra high-performance liquid chromatography coupled with tandem high resolution mass spectrometry, in order to identify compounds responsible for respective bioactivity.

Název v anglickém jazyce

Bioprospecting of Artemisia scoparia, Panax ginseng and Phellodendron sp.

Popis výsledku anglicky

Many herbal extracts are known to contain biologically active molecules, some of which find uses in pharmaceuticals production. In the recent years, substances with a potential to inhibit lipolysis are being searched. One of potential applications is a treatment of obstructive sleep apnea, chronic disease which causes hypoxia and disrupts adipocyte metabolism. The aim of our study was to isolate and identify novel bioactive secondary metabolites from Artemisia scoparia, Panax ginseng and Phellodendron sp., which are presumed to contain lipolysis inhibitors. To obtain the entire set of compounds with different polarities, three types of extracts were prepared from each dried plant: aqueous, methanolic and dichloromethane extracts. These extracts were then fractionated using high performance liquid chromatography Waters AutoPurification System coupled to single quadrupole mass spectrometry detector (QDa). Nine sets of fractions were collected and their antilipolytic activity was measured. An enzymatic assay based on measurement of released free glycerol concentration was employed both for spontaneous and isoprenaline-induced lipolysis. Significant fractions with the highest inhibitory activity were further analyzed using ultra high-performance liquid chromatography coupled with tandem high resolution mass spectrometry, in order to identify compounds responsible for respective bioactivity.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/NU21-01-00259" target="_blank" >NU21-01-00259: Nové inhibitory lipolýzy v léčbě poruch metabolismu lipidů a glukózy při syndromu obstrukční spánkové apnoe</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů