Metabolomic and transcriptomic analyses identify external conditions and key genes underlying high levels of toxic glycoalkaloids in tubers of stress-sensitive potato cultivars

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F23%3A43928180" target="_blank" >RIV/60461373:22330/23:43928180 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1210850/full#h9" target="_blank" >https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1210850/full#h9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2023.1210850" target="_blank" >10.3389/fpls.2023.1210850</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Metabolomic and transcriptomic analyses identify external conditions and key genes underlying high levels of toxic glycoalkaloids in tubers of stress-sensitive potato cultivars

Popis výsledku v původním jazyce

Introduction: High levels of toxic steroidal glycoalkaloids (SGAs) in potato tubers constitute a recognized food quality problem. Tuber SGA levels vary between potato cultivars and can increase after post-harvest stresses such as wounding and light exposure. A few cultivars, e.g., ‘Magnum Bonum’ and ‘Lenape,’ have been withdrawn from commercial sales due to excessive SGA levels during some cultivation years. However, these sudden SGA increases are diffucult to predict, and their causes are not understood. To identify external and genetic factors that underlie sudden SGA increases in certain potato cultivars, we have here in a 2-year study investigated ‘Magnum Bonum’ and five additional table potato cultivars for their SGA levels after wounding and light exposure. Results and methods: Results showed that ‘Magnum Bonum’ has an unusual strong SGA response to light exposure, but not to wounding, whereas ‘Bintje’ displayed an opposite regulation. Levels of calystegine alkaloids were not significantly altered by treatments, implicating independent metabolic regulation of SGA and calystegine levels also under conditions of high SGA accumulation. Metabolomic and transcriptomic analyses identified a small number of key genes whose expression correlated with SGA differences between cultivars. Overexpression of two key genes in transgenic low-SGA potato cultivars increased their leaf SGA levels significantly. Discussion: The results show that a strong response to light can underlie the SGA peaks that occasionally occur in certain potato cultivars and indicate that a between-cultivar variation in the expression of single SGA key genes can account for cultivar SGA differerences. We propose that current attempts to mitigate the SGA hazard will benefit from an increased consideration of cultivar-dependent SGA responses to post-harvest conditions, particularly light exposure. The identified key SGA genes can now be used as a molecular tool in this work. Copyright © 2023 Merino, Guasca, Krmela, Arif, Ali, Westerberg, Jalmi, Hajslova, Schulzova and Sitbon.

Název v anglickém jazyce

Metabolomic and transcriptomic analyses identify external conditions and key genes underlying high levels of toxic glycoalkaloids in tubers of stress-sensitive potato cultivars

Popis výsledku anglicky

Introduction: High levels of toxic steroidal glycoalkaloids (SGAs) in potato tubers constitute a recognized food quality problem. Tuber SGA levels vary between potato cultivars and can increase after post-harvest stresses such as wounding and light exposure. A few cultivars, e.g., ‘Magnum Bonum’ and ‘Lenape,’ have been withdrawn from commercial sales due to excessive SGA levels during some cultivation years. However, these sudden SGA increases are diffucult to predict, and their causes are not understood. To identify external and genetic factors that underlie sudden SGA increases in certain potato cultivars, we have here in a 2-year study investigated ‘Magnum Bonum’ and five additional table potato cultivars for their SGA levels after wounding and light exposure. Results and methods: Results showed that ‘Magnum Bonum’ has an unusual strong SGA response to light exposure, but not to wounding, whereas ‘Bintje’ displayed an opposite regulation. Levels of calystegine alkaloids were not significantly altered by treatments, implicating independent metabolic regulation of SGA and calystegine levels also under conditions of high SGA accumulation. Metabolomic and transcriptomic analyses identified a small number of key genes whose expression correlated with SGA differences between cultivars. Overexpression of two key genes in transgenic low-SGA potato cultivars increased their leaf SGA levels significantly. Discussion: The results show that a strong response to light can underlie the SGA peaks that occasionally occur in certain potato cultivars and indicate that a between-cultivar variation in the expression of single SGA key genes can account for cultivar SGA differerences. We propose that current attempts to mitigate the SGA hazard will benefit from an increased consideration of cultivar-dependent SGA responses to post-harvest conditions, particularly light exposure. The identified key SGA genes can now be used as a molecular tool in this work. Copyright © 2023 Merino, Guasca, Krmela, Arif, Ali, Westerberg, Jalmi, Hajslova, Schulzova and Sitbon.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

—

Svazek periodika

14

Číslo periodika v rámci svazku

04 October 2023

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

16

Strana od-do

—

Kód UT WoS článku

001085027900001

EID výsledku v databázi Scopus

2-s2.0-85174598783