Preparation of galactooligosaccharides with fucose using β-galactosidase.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F24%3A43930954" target="_blank" >RIV/60461373:22330/24:43930954 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of galactooligosaccharides with fucose using β-galactosidase.

Popis výsledku v původním jazyce

Galactooligosaccharides (GOS) are prebiotics that selectively support the growth of probiotic bacteria and thus positively affect gut health, the immune system, metabolism and can contribute to the prevention of certain diseases. Fucose is richly represented in the oligosaccharides of breast milk or in the polysaccharides of seaweed and has potential for application in functional foods. The aim of the work was to verify the preparation process of oligosaccharides from lactose and fucose by an enzymatic reaction catalyzed by β-galactosidase.Lactose and fucose was mixed in ratio weight range from 20:0 to 20:5 in phosphate buffer in pH range 4.5 to 8.0. Enzyme preparation Danisco® Nurica™ was added (1.3 mL per 100 mL of reaction mixture) at 37 °C. The reaction products were analyzed by HPLC on Hypercarb column and ELS detection. GOS were purified on active carbon Norit DX Ultra.Maximal amount of fucose was incorpotated in to GOS at lactose:fucose ratio 20:4, at pH 5.5 after 60 min reaction. After stepwise extraction of GOS by increased concentration of ethanol GOS preparation containing 38.9 % of GOS was obtained. The GOS preparation consisted mainly of tri- and tetrasaccharides and contained a small amount of di- and pentasaccharides. No monosaccharide were in GOS preparation after purification. The differences in the chromatograms of the reaction mixture with and without fucose show that a smaller amount of oligosaccharides with fucose is formedduring the reaction.It follows from the obtained results that with the help of β-galactosidase with high transgalactosylation activity, a GOS mixture can be prepared, which contains a smaller amount of oligosaccharides with fucose.

Název v anglickém jazyce

Preparation of galactooligosaccharides with fucose using β-galactosidase.

Popis výsledku anglicky

Galactooligosaccharides (GOS) are prebiotics that selectively support the growth of probiotic bacteria and thus positively affect gut health, the immune system, metabolism and can contribute to the prevention of certain diseases. Fucose is richly represented in the oligosaccharides of breast milk or in the polysaccharides of seaweed and has potential for application in functional foods. The aim of the work was to verify the preparation process of oligosaccharides from lactose and fucose by an enzymatic reaction catalyzed by β-galactosidase.Lactose and fucose was mixed in ratio weight range from 20:0 to 20:5 in phosphate buffer in pH range 4.5 to 8.0. Enzyme preparation Danisco® Nurica™ was added (1.3 mL per 100 mL of reaction mixture) at 37 °C. The reaction products were analyzed by HPLC on Hypercarb column and ELS detection. GOS were purified on active carbon Norit DX Ultra.Maximal amount of fucose was incorpotated in to GOS at lactose:fucose ratio 20:4, at pH 5.5 after 60 min reaction. After stepwise extraction of GOS by increased concentration of ethanol GOS preparation containing 38.9 % of GOS was obtained. The GOS preparation consisted mainly of tri- and tetrasaccharides and contained a small amount of di- and pentasaccharides. No monosaccharide were in GOS preparation after purification. The differences in the chromatograms of the reaction mixture with and without fucose show that a smaller amount of oligosaccharides with fucose is formedduring the reaction.It follows from the obtained results that with the help of β-galactosidase with high transgalactosylation activity, a GOS mixture can be prepared, which contains a smaller amount of oligosaccharides with fucose.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK22010186" target="_blank" >QK22010186: Postbiotika, bakteriální exopolysacharidy a nové oligosacharidy pro funkční synbiotické fermentované výrobky</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů