Structure and strain specificity for polysaccharides from king oyster mushroom (Pleurotus eryngii) fruiting bodies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F25%3A43930800" target="_blank" >RIV/60461373:22330/25:43930800 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/25:102040

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0141813024100979" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0141813024100979</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2024.139286" target="_blank" >10.1016/j.ijbiomac.2024.139286</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structure and strain specificity for polysaccharides from king oyster mushroom (Pleurotus eryngii) fruiting bodies

Popis výsledku v původním jazyce

King oyster mushroom Pleurotus eryngii is cultivated worldwide for culinary and to improve human health. However, the potential of some Mediterranean representatives of this species is still not evaluated. This work focuses on the study of polysaccharides from fruiting bodies of two Tunisian strains, P. eryngii var. elaeoselini and P. eryngii var. ferulae, and, for comparison, one deposited P. eryngii originated from Korea. Polysaccharides were successively extracted with hot water using microwave heating and 1 mol L−1 aqueous sodium hydroxide. The crude hot water extracts were purified by treating them with proteolytic enzymes, and the alkaline extracts were purified by re-dissolving with dimethyl sulphoxide. In both cases, a decrease or removal of proteins was detected. Glucans predominated in all these products; the insoluble parts also contained chitin. The purified hot water extracts contained glycogen, β-D-glucans and mannogalactan. Branching (1 → 3)(1 → 6)-β-D-glucan was the major polysaccharide in the alkali-soluble fractions, while (1 → 3)-α-D-glucan was only a minor component. The Tunisian strains demonstrated a higher proportion of water-soluble polysaccharides, compared to the alkaline soluble ones, and more β-D-glucan in the insoluble chitin-glucan complexes. Fruiting body proteins of these strains are more available for solubilisation and enzymatic or alkaline degradation and, thus, may have higher nutritional value than those of the reference strain. As a source of proteins or polysaccharides, the Tunisian endemic P. eryngii strains of this study are promising for the domestication and cultivation of fruiting bodies for gastronomic purposes in the North African region. © 2024

Název v anglickém jazyce

Structure and strain specificity for polysaccharides from king oyster mushroom (Pleurotus eryngii) fruiting bodies

Popis výsledku anglicky

King oyster mushroom Pleurotus eryngii is cultivated worldwide for culinary and to improve human health. However, the potential of some Mediterranean representatives of this species is still not evaluated. This work focuses on the study of polysaccharides from fruiting bodies of two Tunisian strains, P. eryngii var. elaeoselini and P. eryngii var. ferulae, and, for comparison, one deposited P. eryngii originated from Korea. Polysaccharides were successively extracted with hot water using microwave heating and 1 mol L−1 aqueous sodium hydroxide. The crude hot water extracts were purified by treating them with proteolytic enzymes, and the alkaline extracts were purified by re-dissolving with dimethyl sulphoxide. In both cases, a decrease or removal of proteins was detected. Glucans predominated in all these products; the insoluble parts also contained chitin. The purified hot water extracts contained glycogen, β-D-glucans and mannogalactan. Branching (1 → 3)(1 → 6)-β-D-glucan was the major polysaccharide in the alkali-soluble fractions, while (1 → 3)-α-D-glucan was only a minor component. The Tunisian strains demonstrated a higher proportion of water-soluble polysaccharides, compared to the alkaline soluble ones, and more β-D-glucan in the insoluble chitin-glucan complexes. Fruiting body proteins of these strains are more available for solubilisation and enzymatic or alkaline degradation and, thus, may have higher nutritional value than those of the reference strain. As a source of proteins or polysaccharides, the Tunisian endemic P. eryngii strains of this study are promising for the domestication and cultivation of fruiting bodies for gastronomic purposes in the North African region. © 2024

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/QK1910209" target="_blank" >QK1910209: Výzkum a vývoj produkce léčivých hub v ČR a jejich inovativní aplikace ve funkčních potravinách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

1879-0003

Svazek periodika

295

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

17

Strana od-do

nestránkováno

Kód UT WoS článku

001409300800001

EID výsledku v databázi Scopus

2-s2.0-85214334994