GC–MS strategy for a comprehensive assessment of cannabis metabolome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F25%3A43931830" target="_blank" >RIV/60461373:22330/25:43931830 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0026265X25004163?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X25004163?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2025.113062" target="_blank" >10.1016/j.microc.2025.113062</a>

Jazyk výsledku

angličtina

Název v původním jazyce

GC–MS strategy for a comprehensive assessment of cannabis metabolome

Popis výsledku v původním jazyce

Cannabis is a very complex plant that provides several potential health benefits. However, these benefits strongly depend on the composition of the present compounds. In this study, we tested and critically evaluated two different sample preparation strategies (with/without derivatization employing N-Methyl-N-(trimethylsilyl)trifluoroacetamide, MSTFA, as a silylating agent) followed by gas chromatography coupled to mass spectrometry (GC–MS). Subsequently, we applied these methods to investigate the metabolome of two chemotypes, including five different varieties of Cannabis. The chemometric methods, such as principal component analysis (PCA) and partial least squares discriminative analysis (PLS-DA), were employed to provide the possibility of distinguishing cannabis varieties based on their metabolome. In the next step, significant compounds (‘markers’) for each variety (or varieties) were found and identified. For the identification of ‘markers’, the NIST library, in combination with an ‘in house’ developed database containing high-resolution mass spectra of 26 neutral cannabinoids and 38 MSTFA derivates, was used. © 2025

Název v anglickém jazyce

GC–MS strategy for a comprehensive assessment of cannabis metabolome

Popis výsledku anglicky

Cannabis is a very complex plant that provides several potential health benefits. However, these benefits strongly depend on the composition of the present compounds. In this study, we tested and critically evaluated two different sample preparation strategies (with/without derivatization employing N-Methyl-N-(trimethylsilyl)trifluoroacetamide, MSTFA, as a silylating agent) followed by gas chromatography coupled to mass spectrometry (GC–MS). Subsequently, we applied these methods to investigate the metabolome of two chemotypes, including five different varieties of Cannabis. The chemometric methods, such as principal component analysis (PCA) and partial least squares discriminative analysis (PLS-DA), were employed to provide the possibility of distinguishing cannabis varieties based on their metabolome. In the next step, significant compounds (‘markers’) for each variety (or varieties) were found and identified. For the identification of ‘markers’, the NIST library, in combination with an ‘in house’ developed database containing high-resolution mass spectra of 26 neutral cannabinoids and 38 MSTFA derivates, was used. © 2025

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MICROCHEMICAL JOURNAL

ISSN

0026-265X

e-ISSN

—

Svazek periodika

210

Číslo periodika v rámci svazku

MAR 2025

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

nestránkováno

Kód UT WoS článku

001428980700001

EID výsledku v databázi Scopus

2-s2.0-85217803852