Innovative antifungal strategies: enhanced biofilm inhibition of Candida albicans by a modified tea tree oil formulation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F25%3A43932151" target="_blank" >RIV/60461373:22330/25:43932151 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1518598/full" target="_blank" >https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1518598/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmicb.2024.1518598" target="_blank" >10.3389/fmicb.2024.1518598</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Innovative antifungal strategies: enhanced biofilm inhibition of Candida albicans by a modified tea tree oil formulation

Popis výsledku v původním jazyce

Introduction Candida albicans is a significant human pathogen with the ability to form biofilms, a critical factor in its resistance to antifungal treatments. This study aims to evaluate the antifungal activity and biofilm inhibition potential of Tea Tree Oil (TTO) derived from Melaleuca alternifolia cultivated in Vietnam.Methods The antifungal activity of TTO was assessed by determining the Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), Minimum Biofilm Inhibitory Concentration (MBIC), and Minimum Biofilm Eradication Concentration (MBEC) using broth dilution methods. The experiments were conducted on C. albicans in both planktonic and biofilm states across concentrations ranging from 0.1 mu L/mL to 10 mu L/mL.Results TTO demonstrated significant antifungal efficacy, with a MIC of 0.1 mu L/mL (similar to 91.217 mu g/mL) and an MFC of 10 mu L/mL (similar to 9121.7 mu g/mL). It effectively inhibited biofilm formation with a recorded MBIC of 2 mu L/mL (similar to 1824.34 mu g/mL). However, MBEC values were not determinable as the concentrations tested did not achieve the eradication of more than 50% of mature biofilm within the experimental conditions.Discussion These findings highlight TTO as a promising natural antifungal agent with strong biofilm-inhibitory properties. However, its limited efficacy in eradicating mature biofilms underscores the need for further studies, potentially involving higher concentrations or synergistic combinations with conventional antifungal agents.

Název v anglickém jazyce

Innovative antifungal strategies: enhanced biofilm inhibition of Candida albicans by a modified tea tree oil formulation

Popis výsledku anglicky

Introduction Candida albicans is a significant human pathogen with the ability to form biofilms, a critical factor in its resistance to antifungal treatments. This study aims to evaluate the antifungal activity and biofilm inhibition potential of Tea Tree Oil (TTO) derived from Melaleuca alternifolia cultivated in Vietnam.Methods The antifungal activity of TTO was assessed by determining the Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), Minimum Biofilm Inhibitory Concentration (MBIC), and Minimum Biofilm Eradication Concentration (MBEC) using broth dilution methods. The experiments were conducted on C. albicans in both planktonic and biofilm states across concentrations ranging from 0.1 mu L/mL to 10 mu L/mL.Results TTO demonstrated significant antifungal efficacy, with a MIC of 0.1 mu L/mL (similar to 91.217 mu g/mL) and an MFC of 10 mu L/mL (similar to 9121.7 mu g/mL). It effectively inhibited biofilm formation with a recorded MBIC of 2 mu L/mL (similar to 1824.34 mu g/mL). However, MBEC values were not determinable as the concentrations tested did not achieve the eradication of more than 50% of mature biofilm within the experimental conditions.Discussion These findings highlight TTO as a promising natural antifungal agent with strong biofilm-inhibitory properties. However, its limited efficacy in eradicating mature biofilms underscores the need for further studies, potentially involving higher concentrations or synergistic combinations with conventional antifungal agents.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

1664-302X

Svazek periodika

15

Číslo periodika v rámci svazku

15 January 2025

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

nestránkováno

Kód UT WoS článku

001408187300001

EID výsledku v databázi Scopus

2-s2.0-85216320526