Open tubular capillary electrochromatgoraphy of underivatized amino acids using Rh/III tetrakis(phenoxyphenyl)porphrinate as wall modifier

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F03%3A00007431" target="_blank" >RIV/60461373:22340/03:00007431 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Open tubular capillary electrochromatgoraphy of underivatized amino acids using Rh/III tetrakis(phenoxyphenyl)porphrinate as wall modifier

Popis výsledku v původním jazyce

The separation of 17 "common" underivatized amino acids was attempted by open tutular capillary electrochromatography (OT.CEC) in fused-silica capillaries coated with Rh(III) tetrakis(phenoxyphenyl)porphyrinate (Rh(III)TPP(m-OPh)4OAc) using sodium phosphate and Tris-phosphate buffers as background electrolytes (BGEs). The OT-CEC separation of amino acids was compared with that obtained by capillary zone electrophoresis in bare fused-silica capilaries using the same BGEs. The amono acids were not derivatized and the UV-absorption detection was set at 200 nm. Depending on the experimental condition at least 15 amono acids were separated. The best separations were obtained in a Rh(III)TPP(m-OPh)4OAc-coated capilllary in 50 mM Tris-100 mM phosphate bufferat pH 2.25. Separation of the critical triplet val-Ile-Leu was always at least indicated being better at higher BGE concentrations. Regarding the sensitivity of the method, Iower concentration limits of detection (LODs) inf the coated cap

Název v anglickém jazyce

Open tubular capillary electrochromatgoraphy of underivatized amino acids using Rh/III tetrakis(phenoxyphenyl)porphrinate as wall modifier

Popis výsledku anglicky

The separation of 17 "common" underivatized amino acids was attempted by open tutular capillary electrochromatography (OT.CEC) in fused-silica capillaries coated with Rh(III) tetrakis(phenoxyphenyl)porphyrinate (Rh(III)TPP(m-OPh)4OAc) using sodium phosphate and Tris-phosphate buffers as background electrolytes (BGEs). The OT-CEC separation of amino acids was compared with that obtained by capillary zone electrophoresis in bare fused-silica capilaries using the same BGEs. The amono acids were not derivatized and the UV-absorption detection was set at 200 nm. Depending on the experimental condition at least 15 amono acids were separated. The best separations were obtained in a Rh(III)TPP(m-OPh)4OAc-coated capilllary in 50 mM Tris-100 mM phosphate bufferat pH 2.25. Separation of the critical triplet val-Ile-Leu was always at least indicated being better at higher BGE concentrations. Regarding the sensitivity of the method, Iower concentration limits of detection (LODs) inf the coated cap

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F02%2F1467" target="_blank" >GA203/02/1467: Vysokoúčinné analytické a preparativní separace biologicky aktivních peptidů a bílkovin kapilárními a průtokovými elektromigračními metodami</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

990

Číslo periodika v rámci svazku

990

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

159-167

Kód UT WoS článku

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EID výsledku v databázi Scopus

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