Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00053816" target="_blank" >RIV/00216224:14310/11:00053816 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis
Popis výsledku v původním jazyce
Inner surface modification of fused-silica capillaries in capillary zone electrophoresis with an appropriate chemical reagents allow targeted selection of separation system for analytes of interest. Polyethyleneimine (PEI) is polycationic highly branchedpolymer with positively charged primary, secondary and tertiary amino groups in the structure that can be used as fused-silica capillary modifier. Its irreversible adsorption on the silanol groups of the fused-silica capillary surface eliminates negative charge and excess of charged amino groups cause reversion of electroosmotic flow. In our work, we successfully applied polyethyleneimine coated capillaries in analysis of nucleotides and synthetic pentamers. We studied the influence of particular component of Tris/boric acid/EDTA buffer (TBE) on the PEI coated capillary surface and the effect of hydroxyethylcellulose (HEC) and/or B-cyclodextrin on the nucleotide separation. Finally, we analyzed DNA restricted fragments from Nicotiana t
Název v anglickém jazyce
Polyethyleneimine coated capillary in nucleotide and short oligonucleotide analysis
Popis výsledku anglicky
Inner surface modification of fused-silica capillaries in capillary zone electrophoresis with an appropriate chemical reagents allow targeted selection of separation system for analytes of interest. Polyethyleneimine (PEI) is polycationic highly branchedpolymer with positively charged primary, secondary and tertiary amino groups in the structure that can be used as fused-silica capillary modifier. Its irreversible adsorption on the silanol groups of the fused-silica capillary surface eliminates negative charge and excess of charged amino groups cause reversion of electroosmotic flow. In our work, we successfully applied polyethyleneimine coated capillaries in analysis of nucleotides and synthetic pentamers. We studied the influence of particular component of Tris/boric acid/EDTA buffer (TBE) on the PEI coated capillary surface and the effect of hydroxyethylcellulose (HEC) and/or B-cyclodextrin on the nucleotide separation. Finally, we analyzed DNA restricted fragments from Nicotiana t
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/LC06035" target="_blank" >LC06035: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů