Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00016303" target="_blank" >RIV/00216224:14310/06:00016303 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins

Popis výsledku v původním jazyce

The properties and behavior of polyethyleneimine (PEI) covalently coated capillaries with respect to different background electrolytes used in capillary zone electrophoresis (CZE) is described. The coating stability and changes of inner surface charge inthe capillary were followed by electroosmotic flow (EOF) measurement. The main interest was focused onto conjugate bases of carboxylic acids as anionic background electrolyte components (acetate, citrate, malate, malonate, tartrate and succinate). Interesting phenomenon in PEI coated capillaries was observed: the direction (and the magnitude) of EOF depends on the composition of the background electrolyte and at a certain pH it can reversibly change. To explain this behavior, a hypothesis of an ionic complex formation was suggested. With this knowledge, the PEI coated capillary was used for the separation of basic proteins in mentioned background electrolytes. As a model sample, a standard protein mixture of cytochrome c, ribonuclease

Název v anglickém jazyce

Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins

Popis výsledku anglicky

The properties and behavior of polyethyleneimine (PEI) covalently coated capillaries with respect to different background electrolytes used in capillary zone electrophoresis (CZE) is described. The coating stability and changes of inner surface charge inthe capillary were followed by electroosmotic flow (EOF) measurement. The main interest was focused onto conjugate bases of carboxylic acids as anionic background electrolyte components (acetate, citrate, malate, malonate, tartrate and succinate). Interesting phenomenon in PEI coated capillaries was observed: the direction (and the magnitude) of EOF depends on the composition of the background electrolyte and at a certain pH it can reversibly change. To explain this behavior, a hypothesis of an ionic complex formation was suggested. With this knowledge, the PEI coated capillary was used for the separation of basic proteins in mentioned background electrolytes. As a model sample, a standard protein mixture of cytochrome c, ribonuclease

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA305%2F03%2F1100" target="_blank" >GA305/03/1100: Syntéza a studium neuroprotektivních peptidů odvozených od humaninu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

29

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

2234-2240

Kód UT WoS článku

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EID výsledku v databázi Scopus

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