Vibračně a elektronově cirkulárně dichroická a absorpční spektrální studie interakcí DNA s 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porfyrinem

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F05%3A00014756" target="_blank" >RIV/60461373:22340/05:00014756 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Vibrational and Electronic Circular Dichroism and Absorption Spectral Study of the DNA - 5, 10, 15, 20-tetrakis(1-methylpyridinium-4-yl)porphyrin Interaction

Popis výsledku v původním jazyce

Vibrational circular dichroism (VCD), electronic circular dichroism (ECD) and IR and UV-Vis absorption spectra were measured for complexes of DNA with 5, 10, 15, 20-Tetrakis(1-methylpyridinium-4-yl)porphyrin (TMPyP) at the [DNA]/[TMPyP] ratios 2/1 - 10/1. The nitrogen base and phosphate spectral regions in IR and the Soret band and DNA regions were studied in UV-Vis. The VCD spectra indicate the priority of intercalation to external minor groove binding and the saturation of the intercalating sites forthe [DNA]/[TMPyP] = 4/1 - 5/1. The combination of spectroscopic results proved that all three binding modes, intercalation, minor groove binding and external binding to the phosphate backbone, take place for the ratio 3/1. It was shown that the melting point of the DNA - TMPyP complex is increased compared to pure DNA. The relative temperature stability of minor groove binding is smaller than of intercalation. The AT base-pair hydrogen bonds were disrupted at low pH for DNA - TMPyP compl

Název v anglickém jazyce

Vibrational and Electronic Circular Dichroism and Absorption Spectral Study of the DNA - 5, 10, 15, 20-tetrakis(1-methylpyridinium-4-yl)porphyrin Interaction

Popis výsledku anglicky

Vibrational circular dichroism (VCD), electronic circular dichroism (ECD) and IR and UV-Vis absorption spectra were measured for complexes of DNA with 5, 10, 15, 20-Tetrakis(1-methylpyridinium-4-yl)porphyrin (TMPyP) at the [DNA]/[TMPyP] ratios 2/1 - 10/1. The nitrogen base and phosphate spectral regions in IR and the Soret band and DNA regions were studied in UV-Vis. The VCD spectra indicate the priority of intercalation to external minor groove binding and the saturation of the intercalating sites forthe [DNA]/[TMPyP] = 4/1 - 5/1. The combination of spectroscopic results proved that all three binding modes, intercalation, minor groove binding and external binding to the phosphate backbone, take place for the ratio 3/1. It was shown that the melting point of the DNA - TMPyP complex is increased compared to pure DNA. The relative temperature stability of minor groove binding is smaller than of intercalation. The AT base-pair hydrogen bonds were disrupted at low pH for DNA - TMPyP compl

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal Mol.Struct.

ISSN

0022-2860

e-ISSN

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Svazek periodika

748

Číslo periodika v rámci svazku

1-3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

17-25

Kód UT WoS článku

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EID výsledku v databázi Scopus

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