Nové porfyrinové conjugaty s fotodynamickým protinádorovým efektem: účinnost mono- a bis-b-cyclodextrinových derivátů v in vivo a in vitro studiu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F06%3A00016680" target="_blank" >RIV/60461373:22340/06:00016680 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Novel porphyrin conjugates with a potent photodynamic antitumor effect: differential efficacy of mono- and bis-b-cyclodextrin derivatives. In vitro and in vivo

Popis výsledku v původním jazyce

In the present study we investigated photosensitizing properties of two novel mono- and bis-cyclodextrin tetrakis(pentafluorophenyl) porphyrin derivatives in several tumor cell lines and in BALB/c mice bearing subcutaneously transplanted syngeneic mousemammary carcinoma, 4T1. Both studied sensitizers were localized mainly into lysosomes and were found to induce cells death by triggering apoptosis in human leukemic cells HL-60. In 4T1 and other cell lines both apoptotic and necrotic modes of cell deathoccurred dependently on drug and light doses. Mono-cyclodextrin porphyrin derivative, P(b-CD)1, exhibited stronger in vitro photodynamic efficacy than bis-cyclodextrin derivative, P(b-CD)2. However, when their efficacy was evaluated in vivo, P(b-CD)2 displayed faster tumor uptake with maximal accumulation 6 h after application leading to complete and prolonged elimination of subcutaneous tumors within 3 days after irradiation (100 J/cm2). In contrast P(b-CD)1 uptake was slower (48 h) and

Název v anglickém jazyce

Novel porphyrin conjugates with a potent photodynamic antitumor effect: differential efficacy of mono- and bis-b-cyclodextrin derivatives. In vitro and in vivo

Popis výsledku anglicky

In the present study we investigated photosensitizing properties of two novel mono- and bis-cyclodextrin tetrakis(pentafluorophenyl) porphyrin derivatives in several tumor cell lines and in BALB/c mice bearing subcutaneously transplanted syngeneic mousemammary carcinoma, 4T1. Both studied sensitizers were localized mainly into lysosomes and were found to induce cells death by triggering apoptosis in human leukemic cells HL-60. In 4T1 and other cell lines both apoptotic and necrotic modes of cell deathoccurred dependently on drug and light doses. Mono-cyclodextrin porphyrin derivative, P(b-CD)1, exhibited stronger in vitro photodynamic efficacy than bis-cyclodextrin derivative, P(b-CD)2. However, when their efficacy was evaluated in vivo, P(b-CD)2 displayed faster tumor uptake with maximal accumulation 6 h after application leading to complete and prolonged elimination of subcutaneous tumors within 3 days after irradiation (100 J/cm2). In contrast P(b-CD)1 uptake was slower (48 h) and

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA301%2F04%2F1315" target="_blank" >GA301/04/1315: Indukce apoptózy macrocyklickými sloučeninami v nádorových buňkách</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Photochemistry and Photobiology

ISSN

0031-8655

e-ISSN

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Svazek periodika

82

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CA - Kanada

Počet stran výsledku

7

Strana od-do

432-438

Kód UT WoS článku

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EID výsledku v databázi Scopus

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