Vysokoúčinná chromatografie a nukleárně magnetická rezonance při studiu lineárních tetrapeptidů a oktapeptidů obsahujících N-methylované aminokyseliny

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F07%3A00019299" target="_blank" >RIV/60461373:22340/07:00019299 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/07:00087561

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

High-performance liquid chromatography and nuclear magnetic resonance study of linear tetrapeptides and octapeptides containing N-methylated amino acid residues

Popis výsledku v původním jazyce

Chromatographic behavior of a series of N-methylated tetra and octapeptides on a reversed-phase sorbent was studied considering the information obtained on the compounds by NMR. The modified tetrapeptides were derived from GFFY-NH2, GFFF-NH2 and GFFH-NH2primary structures by N-methylation at various peptide bond positions. Similarly, the N-methylated octapeptides were based on TPK(Pac)T C-terminally elongated forms of GFFY and GFFF. It was found that many studied N-methylated peptides provide broad peaks as a consequence of cis/trans isomerism of the R1CO-N(CH3)R2 peptide bond. The extent of the peak spreading was found to be strongly dependent on the following important factors: the nature of the surrounding amino acid residues, the location of the modified peptide bond within the peptide chain, temperature and mobile phase flow-rate. All these aspects were critically evaluated. Nearly complete separation of the individual conformers of GF(NMe)FY-NH2 was obtained applying fast chroma

Název v anglickém jazyce

High-performance liquid chromatography and nuclear magnetic resonance study of linear tetrapeptides and octapeptides containing N-methylated amino acid residues

Popis výsledku anglicky

Chromatographic behavior of a series of N-methylated tetra and octapeptides on a reversed-phase sorbent was studied considering the information obtained on the compounds by NMR. The modified tetrapeptides were derived from GFFY-NH2, GFFF-NH2 and GFFH-NH2primary structures by N-methylation at various peptide bond positions. Similarly, the N-methylated octapeptides were based on TPK(Pac)T C-terminally elongated forms of GFFY and GFFF. It was found that many studied N-methylated peptides provide broad peaks as a consequence of cis/trans isomerism of the R1CO-N(CH3)R2 peptide bond. The extent of the peak spreading was found to be strongly dependent on the following important factors: the nature of the surrounding amino acid residues, the location of the modified peptide bond within the peptide chain, temperature and mobile phase flow-rate. All these aspects were critically evaluated. Nearly complete separation of the individual conformers of GF(NMe)FY-NH2 was obtained applying fast chroma

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1160

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

BE - Belgické království

Počet stran výsledku

9

Strana od-do

128-136

Kód UT WoS článku

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EID výsledku v databázi Scopus

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