Infrared spectroscopic study of the model metal-ligand-antibody systems: What information on the structure and stability of systems can be obtained?

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F12%3A43893316" target="_blank" >RIV/60461373:22340/12:43893316 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.vibspec.2012.02.011" target="_blank" >http://dx.doi.org/10.1016/j.vibspec.2012.02.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vibspec.2012.02.011" target="_blank" >10.1016/j.vibspec.2012.02.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Infrared spectroscopic study of the model metal-ligand-antibody systems: What information on the structure and stability of systems can be obtained?

Popis výsledku v původním jazyce

In this study we investigated the possibility of using infrared spectroscopy for the characterization of the ligand (diethylenetriaminepentaacetic acid, DTPA) in various aqueous media (water and different buffers), the antibody (immunoglobulin G, IgG), the metal-ligand complexes (Y-DTPA and Lu-DTPA) and the metal-ligand-antibody conjugates (Y-DTPA-IgG and Lu-DTPA-IgG). The aim was to describe behaviour of individual components, and to find spectral markers of complexes and/or conjugates formation. The reason of the choice of Lu and Y was the potential use of their radionuclides to form radiopharmaceutical species for nuclear medicine. We performed kinetic measurements to describe the stability/instability of individual systems. Multivariate data analysis allowed finding out spectral regions related to different chemical processes. Hydrolysis effect of the diethylenetriaminepentaacetic acid dianhydride (cDTPAA) in water was confirmed in unbuffered aqueous solution. Nevertheless, the stu

Název v anglickém jazyce

Infrared spectroscopic study of the model metal-ligand-antibody systems: What information on the structure and stability of systems can be obtained?

Popis výsledku anglicky

In this study we investigated the possibility of using infrared spectroscopy for the characterization of the ligand (diethylenetriaminepentaacetic acid, DTPA) in various aqueous media (water and different buffers), the antibody (immunoglobulin G, IgG), the metal-ligand complexes (Y-DTPA and Lu-DTPA) and the metal-ligand-antibody conjugates (Y-DTPA-IgG and Lu-DTPA-IgG). The aim was to describe behaviour of individual components, and to find spectral markers of complexes and/or conjugates formation. The reason of the choice of Lu and Y was the potential use of their radionuclides to form radiopharmaceutical species for nuclear medicine. We performed kinetic measurements to describe the stability/instability of individual systems. Multivariate data analysis allowed finding out spectral regions related to different chemical processes. Hydrolysis effect of the diethylenetriaminepentaacetic acid dianhydride (cDTPAA) in water was confirmed in unbuffered aqueous solution. Nevertheless, the stu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GAP206%2F11%2F0951" target="_blank" >GAP206/11/0951: Vývoj měřicích technik teplotně závislé povrchem zesílené vibrační spektroskopie pro bioanalýzu a farmacii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Vibrational Spectroscopy

ISSN

0924-2031

e-ISSN

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Svazek periodika

61

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

78-84

Kód UT WoS článku

000304686400012

EID výsledku v databázi Scopus

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