Circular dichroism study of the interaction between mutagens and bilirubin bound to different binding sites of serum albumins
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F14%3A43897030" target="_blank" >RIV/60461373:22340/14:43897030 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.saa.2014.01.139" target="_blank" >http://dx.doi.org/10.1016/j.saa.2014.01.139</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.saa.2014.01.139" target="_blank" >10.1016/j.saa.2014.01.139</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Circular dichroism study of the interaction between mutagens and bilirubin bound to different binding sites of serum albumins
Popis výsledku v původním jazyce
Although recent investigations have shown that bilirubin not only has a negative role in the organism but also exhibits significant antimutagenic properties, the mechanisms of interactions between bilirubin and mutagens are not clear. In this study, interaction between bilirubin bound to different binding sites of mammalian serum albumins with structural analogues of the mutagens 2-aminofluorene, 2,7-diaminofluorene and mutagen 2,4,7-trinitrofluorenone were investigated by circular dichroism and absorption spectroscopy. Homological human and bovine serum albumins were used as chiral matrices, which preferentially bind different conformers of bilirubin in the primary binding sites and make it observable by circular dichroism. These molecular systems approximated a real system for the study of mutagens in blood serum. Differences between the interaction of bilirubin bound to primary and to secondary binding sites of serum albumins with mutagens were shown. For bilirubin bound to secondar
Název v anglickém jazyce
Circular dichroism study of the interaction between mutagens and bilirubin bound to different binding sites of serum albumins
Popis výsledku anglicky
Although recent investigations have shown that bilirubin not only has a negative role in the organism but also exhibits significant antimutagenic properties, the mechanisms of interactions between bilirubin and mutagens are not clear. In this study, interaction between bilirubin bound to different binding sites of mammalian serum albumins with structural analogues of the mutagens 2-aminofluorene, 2,7-diaminofluorene and mutagen 2,4,7-trinitrofluorenone were investigated by circular dichroism and absorption spectroscopy. Homological human and bovine serum albumins were used as chiral matrices, which preferentially bind different conformers of bilirubin in the primary binding sites and make it observable by circular dichroism. These molecular systems approximated a real system for the study of mutagens in blood serum. Differences between the interaction of bilirubin bound to primary and to secondary binding sites of serum albumins with mutagens were shown. For bilirubin bound to secondar
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GAP206%2F11%2F0836" target="_blank" >GAP206/11/0836: Strukturní studie potencionálně bioaktivních komplexů žlučových barviv: Vztak k jejich ochranné funkci v organismech</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
ISSN
1386-1425
e-ISSN
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Svazek periodika
126
Číslo periodika v rámci svazku
neuvedeno
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
8
Strana od-do
68-75
Kód UT WoS článku
000335618200010
EID výsledku v databázi Scopus
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