On-chip surface acoustic wave lysis and ion-exchange nanomembrane detection of exosomal RNA for pancreatic cancer study and diagnosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F15%3A43900137" target="_blank" >RIV/60461373:22340/15:43900137 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1039/c5lc00036j" target="_blank" >http://dx.doi.org/10.1039/c5lc00036j</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c5lc00036j" target="_blank" >10.1039/c5lc00036j</a>

Jazyk výsledku

angličtina

Název v původním jazyce

On-chip surface acoustic wave lysis and ion-exchange nanomembrane detection of exosomal RNA for pancreatic cancer study and diagnosis

Popis výsledku v původním jazyce

There has been increasing evidence that micro and messenger RNA derived from exosomes play important roles in pancreatic and other cancers. In this work, a microfluidics-based approach to the analysis of exosomal RNA is presented based on surface acoustic wave (SAW) exosome lysis and ion-exchange nanomembrane RNA sensing performed in conjunction on two separate chips. Using microRNA hsa-miR-550 as a model target and raw cell media from pancreatic cancer cell lines as a biological sample, SAW-based exosome lysis is shown to have a lysis rate of 38%, and an ion-exchange nanomembrane sensor is shown to have a limit of detection of 2 pM, with two decades of linear dynamic range. A universal calibration curve was derived for the membrane sensor and used todetect the target at a concentration of 13 pM in a SAWlysed sample, which translates to 14 target miRNA per exosome from the raw cell media. At a total analysis time of similar to 1.5 h, this approach is a significant improvement over exi

Název v anglickém jazyce

On-chip surface acoustic wave lysis and ion-exchange nanomembrane detection of exosomal RNA for pancreatic cancer study and diagnosis

Popis výsledku anglicky

There has been increasing evidence that micro and messenger RNA derived from exosomes play important roles in pancreatic and other cancers. In this work, a microfluidics-based approach to the analysis of exosomal RNA is presented based on surface acoustic wave (SAW) exosome lysis and ion-exchange nanomembrane RNA sensing performed in conjunction on two separate chips. Using microRNA hsa-miR-550 as a model target and raw cell media from pancreatic cancer cell lines as a biological sample, SAW-based exosome lysis is shown to have a lysis rate of 38%, and an ion-exchange nanomembrane sensor is shown to have a limit of detection of 2 pM, with two decades of linear dynamic range. A universal calibration curve was derived for the membrane sensor and used todetect the target at a concentration of 13 pM in a SAWlysed sample, which translates to 14 target miRNA per exosome from the raw cell media. At a total analysis time of similar to 1.5 h, this approach is a significant improvement over exi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CI - Průmyslová chemie a chemické inženýrství

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Lab on a Chip

ISSN

1473-0197

e-ISSN

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Svazek periodika

15

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

1656-1666

Kód UT WoS článku

000351493100007

EID výsledku v databázi Scopus

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